Euthanization methods influence cytokine mRNA expression levels in age 0 year Oncorhynchus mykiss

L. Bowen; J. D. Kiernan; K. Eder; I. Werner; C. M. Leutenegger; M. A. Adkison; Ronald Hedrick; M. L. Johnson

doi:10.1111/j.1095-8649.2011.03034.x

Euthanization methods influence cytokine mRNA expression levels in age 0 year Oncorhynchus mykiss

L. Bowen, J. D. Kiernan, K. Eder, I. Werner, C. M. Leutenegger, M. A. Adkison, Ronald Hedrick, M. L. Johnson

School of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Significant differences in cytokine transcription were found between Oncorhynchus mykiss euthanized using the pharmacological agents MS-222 v. benzocaine and also when contrasting death induced by carbon dioxide asphyxiation v. physical methods (cervical dislocation). This study highlights the need to consider the potentially confounding effect of euthanization method on gene expression data.

Original language	English (US)
Pages (from-to)	539-545
Number of pages	7
Journal	Journal of Fish Biology
Volume	79
Issue number	2
DOIs	https://doi.org/10.1111/j.1095-8649.2011.03034.x
State	Published - Aug 1 2011

Keywords

Benzocaine
Carbon dioxide asphyxiation
Gene transcription
MS-222
Salmonid

ASJC Scopus subject areas

Aquatic Science
Ecology, Evolution, Behavior and Systematics

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abstract = "Significant differences in cytokine transcription were found between Oncorhynchus mykiss euthanized using the pharmacological agents MS-222 v. benzocaine and also when contrasting death induced by carbon dioxide asphyxiation v. physical methods (cervical dislocation). This study highlights the need to consider the potentially confounding effect of euthanization method on gene expression data.",

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AU - Bowen, L.

AU - Kiernan, J. D.

AU - Eder, K.

AU - Werner, I.

AU - Leutenegger, C. M.

AU - Adkison, M. A.

AU - Hedrick, Ronald

AU - Johnson, M. L.

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AB - Significant differences in cytokine transcription were found between Oncorhynchus mykiss euthanized using the pharmacological agents MS-222 v. benzocaine and also when contrasting death induced by carbon dioxide asphyxiation v. physical methods (cervical dislocation). This study highlights the need to consider the potentially confounding effect of euthanization method on gene expression data.

KW - Benzocaine

KW - Carbon dioxide asphyxiation

KW - Gene transcription

KW - MS-222

KW - Salmonid

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Euthanization methods influence cytokine mRNA expression levels in age 0 year Oncorhynchus mykiss

Abstract

Keywords

ASJC Scopus subject areas

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