European wildcat populations are subdivided into five main biogeographic groups: Consequences of Pleistocene climate changes or recent anthropogenic fragmentation?

Federica Mattucci, Rita Oliveira, Leslie A Lyons, Paulo C. Alves, Ettore Randi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Extant populations of the European wildcat are fragmented across the continent, the likely consequence of recent extirpations due to habitat loss and over-hunting. However, their underlying phylogeographic history has never been reconstructed. For testing the hypothesis that the European wildcat survived the Ice Age fragmented in Mediterranean refuges, we assayed the genetic variation at 31 microsatellites in 668 presumptive European wildcats sampled in 15 European countries. Moreover, to evaluate the extent of subspecies/population divergence and identify eventual wild × domestic cat hybrids, we genotyped 26 African wildcats from Sardinia and North Africa and 294 random-bred domestic cats. Results of multivariate analyses and Bayesian clustering confirmed that the European wild and the domestic cats (plus the African wildcats) belong to two well-differentiated clusters (average FST = 0.159, Rst = 0.392, P > 0.001; Analysis of molecular variance [AMOVA]). We identified from c. 5% to 10% cryptic hybrids in southern and central European populations. In contrast, wild-living cats in Hungary and Scotland showed deep signatures of genetic admixture and introgression with domestic cats. The European wildcats are subdivided into five main genetic clusters (average FST = 0.103, Rst = 0.143, P > 0.001; AMOVA) corresponding to five biogeographic groups, respectively, distributed in the Iberian Peninsula, central Europe, central Germany, Italian Peninsula and the island of Sicily, and in north-eastern Italy and northern Balkan regions (Dinaric Alps). Approximate Bayesian Computation simulations supported late Pleistocene-early Holocene population splittings (from c. 60 k to 10 k years ago), contemporary to the last Ice Age climatic changes. These results provide evidences for wildcat Mediterranean refuges in southwestern Europe, but the evolution history of eastern wildcat populations remains to be clarified. Historical genetic subdivisions suggest conservation strategies aimed at enhancing gene flow through the restoration of ecological corridors within each biogeographic units. Concomitantly, the risk of hybridization with free-ranging domestic cats along corridor edges should be carefully monitored.

Original languageEnglish (US)
Pages (from-to)3-22
Number of pages20
JournalEcology and Evolution
Volume6
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

fragmentation
climate change
Pleistocene
cats
refuge
introgression
habitat loss
history
hunting
subspecies
gene flow
genetic variation
ice
Alps region
divergence
Holocene
biological corridors
Balkans
Sardinia
Sicily

Keywords

  • Felis silvestris
  • ABC simulations
  • Bayesian clustering
  • Conservation genetics
  • Microsatellites
  • Phylogeography
  • Population structure
  • Wild and domestic cat hybridization

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Nature and Landscape Conservation

Cite this

European wildcat populations are subdivided into five main biogeographic groups : Consequences of Pleistocene climate changes or recent anthropogenic fragmentation? / Mattucci, Federica; Oliveira, Rita; Lyons, Leslie A; Alves, Paulo C.; Randi, Ettore.

In: Ecology and Evolution, Vol. 6, No. 1, 01.01.2016, p. 3-22.

Research output: Contribution to journalArticle

@article{fcba2f3b1a2e4f57975e7bf1b69dc1de,
title = "European wildcat populations are subdivided into five main biogeographic groups: Consequences of Pleistocene climate changes or recent anthropogenic fragmentation?",
abstract = "Extant populations of the European wildcat are fragmented across the continent, the likely consequence of recent extirpations due to habitat loss and over-hunting. However, their underlying phylogeographic history has never been reconstructed. For testing the hypothesis that the European wildcat survived the Ice Age fragmented in Mediterranean refuges, we assayed the genetic variation at 31 microsatellites in 668 presumptive European wildcats sampled in 15 European countries. Moreover, to evaluate the extent of subspecies/population divergence and identify eventual wild × domestic cat hybrids, we genotyped 26 African wildcats from Sardinia and North Africa and 294 random-bred domestic cats. Results of multivariate analyses and Bayesian clustering confirmed that the European wild and the domestic cats (plus the African wildcats) belong to two well-differentiated clusters (average FST = 0.159, Rst = 0.392, P > 0.001; Analysis of molecular variance [AMOVA]). We identified from c. 5{\%} to 10{\%} cryptic hybrids in southern and central European populations. In contrast, wild-living cats in Hungary and Scotland showed deep signatures of genetic admixture and introgression with domestic cats. The European wildcats are subdivided into five main genetic clusters (average FST = 0.103, Rst = 0.143, P > 0.001; AMOVA) corresponding to five biogeographic groups, respectively, distributed in the Iberian Peninsula, central Europe, central Germany, Italian Peninsula and the island of Sicily, and in north-eastern Italy and northern Balkan regions (Dinaric Alps). Approximate Bayesian Computation simulations supported late Pleistocene-early Holocene population splittings (from c. 60 k to 10 k years ago), contemporary to the last Ice Age climatic changes. These results provide evidences for wildcat Mediterranean refuges in southwestern Europe, but the evolution history of eastern wildcat populations remains to be clarified. Historical genetic subdivisions suggest conservation strategies aimed at enhancing gene flow through the restoration of ecological corridors within each biogeographic units. Concomitantly, the risk of hybridization with free-ranging domestic cats along corridor edges should be carefully monitored.",
keywords = "Felis silvestris, ABC simulations, Bayesian clustering, Conservation genetics, Microsatellites, Phylogeography, Population structure, Wild and domestic cat hybridization",
author = "Federica Mattucci and Rita Oliveira and Lyons, {Leslie A} and Alves, {Paulo C.} and Ettore Randi",
year = "2016",
month = "1",
day = "1",
doi = "10.1002/ece3.1815",
language = "English (US)",
volume = "6",
pages = "3--22",
journal = "Ecology and Evolution",
issn = "2045-7758",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - European wildcat populations are subdivided into five main biogeographic groups

T2 - Consequences of Pleistocene climate changes or recent anthropogenic fragmentation?

AU - Mattucci, Federica

AU - Oliveira, Rita

AU - Lyons, Leslie A

AU - Alves, Paulo C.

AU - Randi, Ettore

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Extant populations of the European wildcat are fragmented across the continent, the likely consequence of recent extirpations due to habitat loss and over-hunting. However, their underlying phylogeographic history has never been reconstructed. For testing the hypothesis that the European wildcat survived the Ice Age fragmented in Mediterranean refuges, we assayed the genetic variation at 31 microsatellites in 668 presumptive European wildcats sampled in 15 European countries. Moreover, to evaluate the extent of subspecies/population divergence and identify eventual wild × domestic cat hybrids, we genotyped 26 African wildcats from Sardinia and North Africa and 294 random-bred domestic cats. Results of multivariate analyses and Bayesian clustering confirmed that the European wild and the domestic cats (plus the African wildcats) belong to two well-differentiated clusters (average FST = 0.159, Rst = 0.392, P > 0.001; Analysis of molecular variance [AMOVA]). We identified from c. 5% to 10% cryptic hybrids in southern and central European populations. In contrast, wild-living cats in Hungary and Scotland showed deep signatures of genetic admixture and introgression with domestic cats. The European wildcats are subdivided into five main genetic clusters (average FST = 0.103, Rst = 0.143, P > 0.001; AMOVA) corresponding to five biogeographic groups, respectively, distributed in the Iberian Peninsula, central Europe, central Germany, Italian Peninsula and the island of Sicily, and in north-eastern Italy and northern Balkan regions (Dinaric Alps). Approximate Bayesian Computation simulations supported late Pleistocene-early Holocene population splittings (from c. 60 k to 10 k years ago), contemporary to the last Ice Age climatic changes. These results provide evidences for wildcat Mediterranean refuges in southwestern Europe, but the evolution history of eastern wildcat populations remains to be clarified. Historical genetic subdivisions suggest conservation strategies aimed at enhancing gene flow through the restoration of ecological corridors within each biogeographic units. Concomitantly, the risk of hybridization with free-ranging domestic cats along corridor edges should be carefully monitored.

AB - Extant populations of the European wildcat are fragmented across the continent, the likely consequence of recent extirpations due to habitat loss and over-hunting. However, their underlying phylogeographic history has never been reconstructed. For testing the hypothesis that the European wildcat survived the Ice Age fragmented in Mediterranean refuges, we assayed the genetic variation at 31 microsatellites in 668 presumptive European wildcats sampled in 15 European countries. Moreover, to evaluate the extent of subspecies/population divergence and identify eventual wild × domestic cat hybrids, we genotyped 26 African wildcats from Sardinia and North Africa and 294 random-bred domestic cats. Results of multivariate analyses and Bayesian clustering confirmed that the European wild and the domestic cats (plus the African wildcats) belong to two well-differentiated clusters (average FST = 0.159, Rst = 0.392, P > 0.001; Analysis of molecular variance [AMOVA]). We identified from c. 5% to 10% cryptic hybrids in southern and central European populations. In contrast, wild-living cats in Hungary and Scotland showed deep signatures of genetic admixture and introgression with domestic cats. The European wildcats are subdivided into five main genetic clusters (average FST = 0.103, Rst = 0.143, P > 0.001; AMOVA) corresponding to five biogeographic groups, respectively, distributed in the Iberian Peninsula, central Europe, central Germany, Italian Peninsula and the island of Sicily, and in north-eastern Italy and northern Balkan regions (Dinaric Alps). Approximate Bayesian Computation simulations supported late Pleistocene-early Holocene population splittings (from c. 60 k to 10 k years ago), contemporary to the last Ice Age climatic changes. These results provide evidences for wildcat Mediterranean refuges in southwestern Europe, but the evolution history of eastern wildcat populations remains to be clarified. Historical genetic subdivisions suggest conservation strategies aimed at enhancing gene flow through the restoration of ecological corridors within each biogeographic units. Concomitantly, the risk of hybridization with free-ranging domestic cats along corridor edges should be carefully monitored.

KW - Felis silvestris

KW - ABC simulations

KW - Bayesian clustering

KW - Conservation genetics

KW - Microsatellites

KW - Phylogeography

KW - Population structure

KW - Wild and domestic cat hybridization

UR - http://www.scopus.com/inward/record.url?scp=84954545636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84954545636&partnerID=8YFLogxK

U2 - 10.1002/ece3.1815

DO - 10.1002/ece3.1815

M3 - Article

AN - SCOPUS:84954545636

VL - 6

SP - 3

EP - 22

JO - Ecology and Evolution

JF - Ecology and Evolution

SN - 2045-7758

IS - 1

ER -