Etiology of sarcoplasmic reticulum calcium release channel lesions in doxorubicin-induced cardiomyopathy

Isaac N Pessah, Mary J. Schiedt, Mostafa A. Shalaby, Matthew Mack, Shri N. Giri

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Alterations in the native function of the ryanodine-sensitive Ca2+ release channel complex of sarcoplasmic reticulum (SR) isolated from rat cardiac ventricles during acute and chronic exposure to doxorubicin are examined. Compared to control SR, actively loaded SR from animals exposed to a single intravenous dose of doxorubicin exhibit faster rates of doxorubicin-induced Ca2+ release and the occupancy of [3H]ryanodine is significantly enhanced with subsequent exposure of SR membranes to doxorubicin in vitro. One week after acute exposure to doxorubicin in vitro, the EC50 for activation of the binding of [3H]ryanodine by Ca2+ is not significantly different from control SR. However, the persistence of doxorubicin-sensitized SR channels appears to be latent since repeated exposure to doxorubicin in vitro significantly enhances receptor occupancy in SR obtained from the treated rats compared to control SR. Ryanodine receptors from rats chronically exposed to doxorubicin consistently exhibit a higher sensitivity to activation Ca2+ which persists at least 4 weeks following the last injection of drug. Chronic exposure produces a concomitant in the capacity of [3H]ryanodine binding sites. The marked decrease in receptor density observed with SR from doxorubicin-treated rats coincides with significant reduction in body weight, suggesting a possible influence of nutrition. However, sodium dodecyl sulfate polyacrylamide electrophoresis indicates no significant loss of the high molecular weight subunit of the ryanodine receptor, suggesting that loss of [3H]ryanodine-binding capacity may be the result of progressive and permanent channel of desensitization. Consistent with desensitization. Consistent with desensitized receptors, membrane vesicles prepared from rats chronically exposed to doxorubicin take up significantly more Ca2+ and exhibit significantly reduced rates of doxorubicin or Ca2+/ryanodine induced Ca2+ release. The data demonstrates (i) doxorubicin inflicts cumulative SR channel lesions in vivo, (ii) a persistent sensitization of the SR channel to activation by Ca2+ and (iii) a significant and apparently irreversible reduction in the number of functional channel complexes.

Original languageEnglish (US)
Pages (from-to)189-206
Number of pages18
JournalToxicology
Volume72
Issue number2
DOIs
StatePublished - 1992

Fingerprint

Sarcoplasmic Reticulum
Calcium Channels
Cardiomyopathies
Doxorubicin
Calcium
Ryanodine
Rats
Ryanodine Receptor Calcium Release Channel
Chemical activation
Membranes
Nutrition
Electrophoresis
Sodium Dodecyl Sulfate
Heart Ventricles
Animals
Molecular Weight
Molecular weight
Binding Sites
Body Weight

Keywords

  • Adriamycin
  • Cardiomyopathy
  • Doxorubicin
  • Ryanodine receptor
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Toxicology

Cite this

Etiology of sarcoplasmic reticulum calcium release channel lesions in doxorubicin-induced cardiomyopathy. / Pessah, Isaac N; Schiedt, Mary J.; Shalaby, Mostafa A.; Mack, Matthew; Giri, Shri N.

In: Toxicology, Vol. 72, No. 2, 1992, p. 189-206.

Research output: Contribution to journalArticle

Pessah, Isaac N ; Schiedt, Mary J. ; Shalaby, Mostafa A. ; Mack, Matthew ; Giri, Shri N. / Etiology of sarcoplasmic reticulum calcium release channel lesions in doxorubicin-induced cardiomyopathy. In: Toxicology. 1992 ; Vol. 72, No. 2. pp. 189-206.
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