Ethanol-induced changes in hepatic free radical defense mechanisms and fatty-acid composition in the miniature pig

Sheri Zidenberg-Cherr, Katherine L. Olin, Jesus Villanueva, Anna Tang, Stephen D. Phinney, Charles H. Halsted, Carl L Keen

Research output: Contribution to journalArticle

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Abstract

In the miniature pig, ethanol consumption has been reported to induce alterations in hepatic antioxidant defense capacity, which could result in increased risk of peroxidative damage. However, ethanol may also induce changes in membrane fatty acid composition, which could reduce the risk of peroxidative damage. This study examined lipid peroxidation, antioxidant defense and fatty acid composition in livers from miniature pigs fed ethanol in diets containing 12% of their calories as fat for 20 mo. After 12 and 20 mo of feeding, ethanol-fed pigs had higher hepatic manganese-superoxide dismutase activity, lower hepatic copper concentrations and low hepatic copper-zinc-superoxide dismutase and glutathione peroxidase activities compared with controls. Lipid peroxidation as assessed by thiobarbituric acid reacting substance assay was lower in liver homogenate and mitochondrial and microsomal fractions from ethanol-fed pigs than in controls. The percentage contribution of highly unsaturated fatty acids to total fatty acids in liver homogenates (after 12 mo of feeding) and microsome fractions (after 20 mo of feeding) was lower in the ethanol-fed pigs than in the controls, resulting in a lower peroxidizability index. Ethanol-fed pigs had minimal or no hepatic damage as assessed by histological methods. We suggest that the relative resistance of microsomes to lipid peroxidation is due to the lower peroxidizability index in the ethanol-fed pigs and may account in part for the absence of significant histopathological findings after 20 mo of ethanol feeding.

Original languageEnglish (US)
Pages (from-to)1185-1192
Number of pages8
JournalHepatology
Volume13
Issue number6
StatePublished - Jun 1991

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Free Radicals
Ethanol
Swine
Fatty Acids
Liver
Lipid Peroxidation
Microsomes
Superoxide Dismutase
Copper
Antioxidants
Glutathione Peroxidase
Unsaturated Fatty Acids
Zinc
Fats
Diet
Membranes

ASJC Scopus subject areas

  • Hepatology

Cite this

Zidenberg-Cherr, S., Olin, K. L., Villanueva, J., Tang, A., Phinney, S. D., Halsted, C. H., & Keen, C. L. (1991). Ethanol-induced changes in hepatic free radical defense mechanisms and fatty-acid composition in the miniature pig. Hepatology, 13(6), 1185-1192.

Ethanol-induced changes in hepatic free radical defense mechanisms and fatty-acid composition in the miniature pig. / Zidenberg-Cherr, Sheri; Olin, Katherine L.; Villanueva, Jesus; Tang, Anna; Phinney, Stephen D.; Halsted, Charles H.; Keen, Carl L.

In: Hepatology, Vol. 13, No. 6, 06.1991, p. 1185-1192.

Research output: Contribution to journalArticle

Zidenberg-Cherr, S, Olin, KL, Villanueva, J, Tang, A, Phinney, SD, Halsted, CH & Keen, CL 1991, 'Ethanol-induced changes in hepatic free radical defense mechanisms and fatty-acid composition in the miniature pig', Hepatology, vol. 13, no. 6, pp. 1185-1192.
Zidenberg-Cherr S, Olin KL, Villanueva J, Tang A, Phinney SD, Halsted CH et al. Ethanol-induced changes in hepatic free radical defense mechanisms and fatty-acid composition in the miniature pig. Hepatology. 1991 Jun;13(6):1185-1192.
Zidenberg-Cherr, Sheri ; Olin, Katherine L. ; Villanueva, Jesus ; Tang, Anna ; Phinney, Stephen D. ; Halsted, Charles H. ; Keen, Carl L. / Ethanol-induced changes in hepatic free radical defense mechanisms and fatty-acid composition in the miniature pig. In: Hepatology. 1991 ; Vol. 13, No. 6. pp. 1185-1192.
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