Ethanol as a potential mosquito sample storage medium for RNA preservation [version 1; peer review: 2 approved]

Mirsha G. Torres, Allison M. Weakley, James D. Hibbert, Oscar D. Kirstein, Gregory C Lanzaro, Yoosook Lee

Research output: Contribution to journalArticle

Abstract

Sample storage for downstream RNA analysis can be challenging in some field settings, especially where access to cryogenic materials or refrigeration/freezer facilities are limited. This has limited RNA-based studies on African malaria vectors collected in the field. We evaluated RNA quality after storing mosquito samples in three different sample preservation media over a 4-week period. Storing mosquito specimens in cold (4°C) media significantly improved yields of intact RNA. Our results indicate commercially available products perform well in keeping RNA integrity as advertised. Moreover, absolute ethanol may be an economical alternative for sample preservation that can be utilized in some resource-limited settings.

Original languageEnglish (US)
Article number1431
JournalF1000Research
Volume8
DOIs
StatePublished - Jan 1 2019

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Peer Review
Culicidae
Ethanol
RNA
Refrigeration
Cryogenics
Malaria

Keywords

  • Field settings
  • Genetic analysis
  • Mosquito
  • RNA preservation
  • Sample storage

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Ethanol as a potential mosquito sample storage medium for RNA preservation [version 1; peer review : 2 approved]. / Torres, Mirsha G.; Weakley, Allison M.; Hibbert, James D.; Kirstein, Oscar D.; Lanzaro, Gregory C; Lee, Yoosook.

In: F1000Research, Vol. 8, 1431, 01.01.2019.

Research output: Contribution to journalArticle

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