Estrogen restores multisynaptic boutons in the dorsolateral prefrontal cortex while promoting working memory in aged rhesus monkeys

Yuko Hara, Frank Yuk, Rishi Puri, William G.M. Janssen, Peter R. Rapp, John Morrison

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Humans and nonhuman primates are vulnerable to age- and menopause-related decline in working memory, a cognitive function reliant on area 46 of the dorsolateral prefrontal cortex (dlPFC). We showed previously that presynaptic mitochondrial number and morphology inmonkeydlPFC neurons correlate with workingmemoryperformance. The current study tested the hypothesis that the types of synaptic connections these boutons form are altered with aging and menopause in rhesus monkeys and that these metrics may be coupled with mitochondrial measures and working memory. Using serial section electron microscopy, we examined the frequencies and characteristics of nonsynaptic, single-synaptic, and multisynaptic boutons (MSBs) in the dlPFC. In contrast to our previous observations in the monkey hippocampal dentate gyrus, where MSBs comprised~40% of boutons, the vast majority of dlPFC boutons were single-synaptic, whereas MSBs constituted a mere 10%. The frequency of MSBs was not altered by normal aging, but decreased by over 50% with surgical menopause induced by ovariectomy in aged monkeys. Cyclic estradiol treatment in aged ovariectomized animals restored MSB frequencies to levels comparable to young and aged premenopausal monkeys. Notably, the frequency of MSBs positively correlated with working memory scores, as measured by the average accuracy on the delayed response (DR) test. Furthermore, MSB incidence positively correlated with the number of healthy straight mitochondria in dlPFC boutons and inversely correlated with the number of pathological donut-shaped mitochondria. Together, our data suggest that MSBs are coupled to cognitive function and mitochondrial health and are sensitive to estrogen.

Original languageEnglish (US)
Pages (from-to)901-910
Number of pages10
JournalJournal of Neuroscience
Volume36
Issue number3
DOIs
StatePublished - Jan 20 2016

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Prefrontal Cortex
Macaca mulatta
Short-Term Memory
Estrogens
Menopause
Haplorhini
Presynaptic Terminals
Cognition
Mitochondria
Parahippocampal Gyrus
Dentate Gyrus
Ovariectomy
Primates
Estradiol
Electron Microscopy
Neurons
Incidence
Health

Keywords

  • Area 46
  • Delayed response
  • Estradiol
  • Menopause
  • Mitochondria
  • MSB

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Estrogen restores multisynaptic boutons in the dorsolateral prefrontal cortex while promoting working memory in aged rhesus monkeys. / Hara, Yuko; Yuk, Frank; Puri, Rishi; Janssen, William G.M.; Rapp, Peter R.; Morrison, John.

In: Journal of Neuroscience, Vol. 36, No. 3, 20.01.2016, p. 901-910.

Research output: Contribution to journalArticle

Hara, Yuko ; Yuk, Frank ; Puri, Rishi ; Janssen, William G.M. ; Rapp, Peter R. ; Morrison, John. / Estrogen restores multisynaptic boutons in the dorsolateral prefrontal cortex while promoting working memory in aged rhesus monkeys. In: Journal of Neuroscience. 2016 ; Vol. 36, No. 3. pp. 901-910.
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