Abstract
Estrogen receptors (ER) in cholangiocytes of primary biliary cirrhosis (PBC) patients and their relationship with cell proliferation and death were evaluated. Liver biopsies from PBC patients with different histological stages were investigated by immunohistochemistry for ER-α and -β, cytokeratin-19, proliferating cellular nuclear antigen (PCNA), Fas and terminal deoxynucleotide transferase end labelling (TUNEL). Normal livers and livers from primary sclerosing cholangitis and alcoholic cirrhosis were investigated as controls. ER-α and -β were observed in cholangiocytes of PBC patients but not in normal liver. In PBC, positivity for ER-β was high (50-65%) in all histological stages while, positivity for ER-α increased from 1% in stage I to 12% in stage III (positivity correlated and co-localized in the same cell with PCNA). In stage IV of PBC, cholangiocytes were negative for ER-α in association with a lower PCNA positivity and with maximal degree of ductopenia. ER-α positivity in cholangiocytes of PBC patients was markedly lower than primary sclerosing cholangitis and alcoholic cirrhosis. ER are expressed in PBC and other pathologies associated with cholangiocyte proliferation but not in normal subjects. The low expression of ER-α in PBC and their disappearance in the advanced histological stages suggests that an estrogenic deficiency could favour the evolution of this disease toward ductopenia.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 905-912 |
| Number of pages | 8 |
| Journal | Journal of Hepatology |
| Volume | 41 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Gastroenterology
Cite this
Estrogen receptors in cholangiocytes and the progression of primary biliary cirrhosis. / Alvaro, Domenico; Invernizzi, Pietro; Onori, Paolo; Franchitto, Antonio; De Santis, Adriano; Crosignani, Andrea; Sferra, Roberta; Ginanni-Corradini, Stefano; Grazia Mancino, Maria; Maggioni, Marco; Attili, Adolfo F.; Podda, Mauro; Gaudio, Eugenio.
In: Journal of Hepatology, Vol. 41, No. 6, 12.2004, p. 905-912.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Estrogen receptors in cholangiocytes and the progression of primary biliary cirrhosis
AU - Alvaro, Domenico
AU - Invernizzi, Pietro
AU - Onori, Paolo
AU - Franchitto, Antonio
AU - De Santis, Adriano
AU - Crosignani, Andrea
AU - Sferra, Roberta
AU - Ginanni-Corradini, Stefano
AU - Grazia Mancino, Maria
AU - Maggioni, Marco
AU - Attili, Adolfo F.
AU - Podda, Mauro
AU - Gaudio, Eugenio
PY - 2004/12
Y1 - 2004/12
N2 - Estrogen receptors (ER) in cholangiocytes of primary biliary cirrhosis (PBC) patients and their relationship with cell proliferation and death were evaluated. Liver biopsies from PBC patients with different histological stages were investigated by immunohistochemistry for ER-α and -β, cytokeratin-19, proliferating cellular nuclear antigen (PCNA), Fas and terminal deoxynucleotide transferase end labelling (TUNEL). Normal livers and livers from primary sclerosing cholangitis and alcoholic cirrhosis were investigated as controls. ER-α and -β were observed in cholangiocytes of PBC patients but not in normal liver. In PBC, positivity for ER-β was high (50-65%) in all histological stages while, positivity for ER-α increased from 1% in stage I to 12% in stage III (positivity correlated and co-localized in the same cell with PCNA). In stage IV of PBC, cholangiocytes were negative for ER-α in association with a lower PCNA positivity and with maximal degree of ductopenia. ER-α positivity in cholangiocytes of PBC patients was markedly lower than primary sclerosing cholangitis and alcoholic cirrhosis. ER are expressed in PBC and other pathologies associated with cholangiocyte proliferation but not in normal subjects. The low expression of ER-α in PBC and their disappearance in the advanced histological stages suggests that an estrogenic deficiency could favour the evolution of this disease toward ductopenia.
AB - Estrogen receptors (ER) in cholangiocytes of primary biliary cirrhosis (PBC) patients and their relationship with cell proliferation and death were evaluated. Liver biopsies from PBC patients with different histological stages were investigated by immunohistochemistry for ER-α and -β, cytokeratin-19, proliferating cellular nuclear antigen (PCNA), Fas and terminal deoxynucleotide transferase end labelling (TUNEL). Normal livers and livers from primary sclerosing cholangitis and alcoholic cirrhosis were investigated as controls. ER-α and -β were observed in cholangiocytes of PBC patients but not in normal liver. In PBC, positivity for ER-β was high (50-65%) in all histological stages while, positivity for ER-α increased from 1% in stage I to 12% in stage III (positivity correlated and co-localized in the same cell with PCNA). In stage IV of PBC, cholangiocytes were negative for ER-α in association with a lower PCNA positivity and with maximal degree of ductopenia. ER-α positivity in cholangiocytes of PBC patients was markedly lower than primary sclerosing cholangitis and alcoholic cirrhosis. ER are expressed in PBC and other pathologies associated with cholangiocyte proliferation but not in normal subjects. The low expression of ER-α in PBC and their disappearance in the advanced histological stages suggests that an estrogenic deficiency could favour the evolution of this disease toward ductopenia.
UR - http://www.scopus.com/inward/record.url?scp=20844433859&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20844433859&partnerID=8YFLogxK
U2 - 10.1016/j.jhep.2004.08.022
DO - 10.1016/j.jhep.2004.08.022
M3 - Article
VL - 41
SP - 905
EP - 912
JO - Journal of Hepatology
JF - Journal of Hepatology
SN - 0168-8278
IS - 6
ER -