Estrogen receptor expression in serially cultivated rat endometrial cells: Stimulation by forskolin and cholera toxin

R. Heimann, R. H. Rice, M. K. Gross, E. L. Coe

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Serially propagated with 3T3 feeder layer support, epithelial cells derived from normal rat endometrium expressed estrogen receptor activity. Specific binding of 17β-estradiol was in the range of 30-60 fmol/mg of protein and was of high affinity (K(d) = 0.3 nM). A survey of cell lines derived from several other normal epithelia showed that rat vaginal and human cervical cultures also had high-affinity estrogen receptors (6-13 fmol/mg of protein), while rat epidermal and esophageal cells had no detectable activity. In the endometrial cultures, receptor levels were elevated nearly two- to fourfold by cholera toxin or forskolin in the medium. This effect was detectable after 4 hr but not 1 hr after treatment and did not occur in the presence of cycloheximide. We conclude that serially cultivated rat endometrial cells retain hormonal properties expressed in vivo while exhibiting some keratinocyte character. These cells may provide a useful model for study of receptor modulation.

Original languageEnglish (US)
Pages (from-to)197-200
Number of pages4
JournalJournal of Cellular Physiology
Volume123
Issue number2
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

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