Estrogen induces rapid decrease in dendritic thorns of CA3 pyramidal neurons in adult male rat hippocampus

Tomokazu Tsurugizawa, Hideo Mukai, Nobuaki Tanabe, Gen Murakami, Yasushi Hojo, Shiro Kominami, Kenji Mitsuhashi, Yoshimasa Komatsuzaki, John Morrison, William G M Janssen, Tetsuya Kimoto, Suguru Kawato

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Modulation of hippocampal synaptic plasticity by estrogen has been attracting much attention. Thorns of thorny excrescences of CA3 hippocampal neurons are post-synaptic regions whose presynaptic partners are mossy fiber terminals. Here we demonstrated the rapid effect of estradiol on the density of thorns of thorny excrescences, by imaging Lucifer Yellow-injected CA3 neurons in adult male rat hippocampal slices. The application of 1 nM estradiol induced rapid decrease in the density of thorns on pyramidal neurons within 2 h. The estradiol-mediated decrease in the density of thorns was blocked by CNQX (AMPA receptor antagonist) and PD98059 (MAP kinase inhibitor), but not by MK-801 (NMDA receptor antagonist). ERα agonist PPT induced the same suppressive effect as that induced by estradiol on the density of thorns, but ERβ agonist DPN did not affect the density of thorns. Note that a 1 nM estradiol treatment did not affect the density of spines in the stratum radiatum and stratum oriens. A search for synaptic ERα was performed using purified RC-19 antibody. The localization of ERα (67 kDa) in the CA3 mossy fiber terminals and thorns was demonstrated using immunogold electron microscopy. These results imply that estradiol drives the signaling pathway including ERα and MAP kinase.

Original languageEnglish (US)
Pages (from-to)1345-1352
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number4
DOIs
StatePublished - Dec 2 2005
Externally publishedYes

Keywords

  • CA3
  • Estrogen
  • Estrogen receptor
  • Hippocampus
  • Spine
  • Synaptic plasticity
  • Thorny excrescence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Tsurugizawa, T., Mukai, H., Tanabe, N., Murakami, G., Hojo, Y., Kominami, S., Mitsuhashi, K., Komatsuzaki, Y., Morrison, J., Janssen, W. G. M., Kimoto, T., & Kawato, S. (2005). Estrogen induces rapid decrease in dendritic thorns of CA3 pyramidal neurons in adult male rat hippocampus. Biochemical and Biophysical Research Communications, 337(4), 1345-1352. https://doi.org/10.1016/j.bbrc.2005.09.188