Estradiol rapidly rescues synaptic transmission from corticosterone-induced suppression via synaptic/extranuclear steroid receptors in the hippocampus

Yuuki Ooishi, Hideo Mukai, Yasushi Hojo, Gen Murakami, Yoshitaka Hasegawa, Tomu Shindo, John Morrison, Tetsuya Kimoto, Suguru Kawato

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We investigated rapid protection effect by estradiol on corticosterone (CORT)-induced suppression of synaptic transmission. Rapid suppression by 1 μM CORT of long-term potentiation (LTP) at CA3-CA1 synapses was abolished via coperfusion of 1 nM estradiol. N-methyl-D-aspartate (NMDA) receptor-derived field excitatory postsynaptic potential (NMDA-R-fEPSP) was used to analyze the mechanisms of these events. Estradiol abolished CORT-induced suppression of NMDA-R-fEPSP slope. This CORT-induced suppression was abolished by calcineurin inhibitor, and the rescue effect by estradiol on the CORT-induced suppression was inhibited by mitogen-activated protein (MAP) kinase inhibitor. The CORT-induced suppressions of LTP and NMDA-R-fEPSP slope were abolished by glucocorticoid receptor (GR) antagonist, and the restorative effects by estradiol on these processes were mimicked by estrogen receptor α (ERα) and ERβ agonists. Taken together, estradiol rapidly rescued LTP and NMDA-R-fEPSP slope from CORT-induced suppressions. A GR→calcineurin pathway is involved in these suppressive effects. The rescue effects by estradiol are driven via ERα or ERβ→MAP kinase pathway. Synaptic/extranuclear GR, ERα, and ERβ probably participate in these rapid events. Mass-spectrometric analysis determined that acute hippocampal slices used for electrophysiological measurements contained 0.48 nM estradiol less than exogenously applied 1 nM. In vivo physiological level of 8 nM estradiol could protect the intact hippocampus against acute stress-induced neural suppression.

Original languageEnglish (US)
Pages (from-to)926-936
Number of pages11
JournalCerebral Cortex
Volume22
Issue number4
DOIs
StatePublished - Apr 2 2012
Externally publishedYes

Fingerprint

Steroid Receptors
Corticosterone
Synaptic Transmission
Estradiol
Hippocampus
Estrogen Receptors
Long-Term Potentiation
N-Methylaspartate
Glucocorticoid Receptors
Excitatory Postsynaptic Potentials
Protein Kinase Inhibitors
Mitogen-Activated Protein Kinases
N-Methyl-D-Aspartate Receptors
Synapses
Estrogens
Phosphotransferases

Keywords

  • corticosterone
  • estradiol
  • hippocampus
  • LTP
  • NDMA receptors

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

Estradiol rapidly rescues synaptic transmission from corticosterone-induced suppression via synaptic/extranuclear steroid receptors in the hippocampus. / Ooishi, Yuuki; Mukai, Hideo; Hojo, Yasushi; Murakami, Gen; Hasegawa, Yoshitaka; Shindo, Tomu; Morrison, John; Kimoto, Tetsuya; Kawato, Suguru.

In: Cerebral Cortex, Vol. 22, No. 4, 02.04.2012, p. 926-936.

Research output: Contribution to journalArticle

Ooishi, Yuuki ; Mukai, Hideo ; Hojo, Yasushi ; Murakami, Gen ; Hasegawa, Yoshitaka ; Shindo, Tomu ; Morrison, John ; Kimoto, Tetsuya ; Kawato, Suguru. / Estradiol rapidly rescues synaptic transmission from corticosterone-induced suppression via synaptic/extranuclear steroid receptors in the hippocampus. In: Cerebral Cortex. 2012 ; Vol. 22, No. 4. pp. 926-936.
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AU - Hasegawa, Yoshitaka

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