Abstract
To estimate the numbers and volumes of bronchiolar epithelial cells during lung maturation, we examined rabbits at three time points, 30 days gestation and 4 and 17 weeks postnatal age. Morphometric measures (mean caliper diameter, surface area, and volume) of nonciliated and ciliated bronchiolar cell nuclei, using computer modeling from serial sections, showed a significant decrease in nuclear size for both cell types and a significant increase in cell volume for the nonciliated bronchiolar cell during lung maturation. A shape coefficient (β) proved to be the most efficient estimator of the number of cells per unit volume when it was used with estimates of the number of nuclei per unit area and the volumetric density of nuclei. Two-dimensional estimates of bronchiolar epithelial cell abundance (the number of nuclei per unit length or area) significantly underestimated the percentage of nonciliated bronchiolar cells as compared to three-dimensional estimates for rabbits 17 weeks of age. We have shown an inverse relationship between nonciliated and ciliated bronchiolar cell abundance during lung maturation. Nonciliated cells decreased while ciliated cells increased. We have confirmed that cytodifferentiation of the nonciliated bronchiolar cell occurs within the first 4 weeks of postnatal development. The volume of the nonciliated bronchiolar cell increased about twofold during development. Because of the concomitant decrease in nuclear volume, the cytoplasm o the cell showed an even. Within the cytoplasm of the nonciliated bronchiolar cell, glycogen significantly decreased, and agranular endoplasmic reticulum (AER) and mitochondria significantly increased in volume during development. The biosynthesis of AER closely correlated with pharmacological studies of xenobiotic metabolism during rabbit lung maturation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 359-370 |
| Number of pages | 12 |
| Journal | American Journal of Anatomy |
| Volume | 167 |
| Issue number | 3 |
| State | Published - 1983 |
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ASJC Scopus subject areas
- Anatomy
Cite this
Estimation of cell numbers and volumes of bronchiolar epithelium during rabbit lung maturation. / Hyde, D. M.; Plopper, Charles; Kass, Philip H; Alley, J. L.
In: American Journal of Anatomy, Vol. 167, No. 3, 1983, p. 359-370.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Estimation of cell numbers and volumes of bronchiolar epithelium during rabbit lung maturation
AU - Hyde, D. M.
AU - Plopper, Charles
AU - Kass, Philip H
AU - Alley, J. L.
PY - 1983
Y1 - 1983
N2 - To estimate the numbers and volumes of bronchiolar epithelial cells during lung maturation, we examined rabbits at three time points, 30 days gestation and 4 and 17 weeks postnatal age. Morphometric measures (mean caliper diameter, surface area, and volume) of nonciliated and ciliated bronchiolar cell nuclei, using computer modeling from serial sections, showed a significant decrease in nuclear size for both cell types and a significant increase in cell volume for the nonciliated bronchiolar cell during lung maturation. A shape coefficient (β) proved to be the most efficient estimator of the number of cells per unit volume when it was used with estimates of the number of nuclei per unit area and the volumetric density of nuclei. Two-dimensional estimates of bronchiolar epithelial cell abundance (the number of nuclei per unit length or area) significantly underestimated the percentage of nonciliated bronchiolar cells as compared to three-dimensional estimates for rabbits 17 weeks of age. We have shown an inverse relationship between nonciliated and ciliated bronchiolar cell abundance during lung maturation. Nonciliated cells decreased while ciliated cells increased. We have confirmed that cytodifferentiation of the nonciliated bronchiolar cell occurs within the first 4 weeks of postnatal development. The volume of the nonciliated bronchiolar cell increased about twofold during development. Because of the concomitant decrease in nuclear volume, the cytoplasm o the cell showed an even. Within the cytoplasm of the nonciliated bronchiolar cell, glycogen significantly decreased, and agranular endoplasmic reticulum (AER) and mitochondria significantly increased in volume during development. The biosynthesis of AER closely correlated with pharmacological studies of xenobiotic metabolism during rabbit lung maturation.
AB - To estimate the numbers and volumes of bronchiolar epithelial cells during lung maturation, we examined rabbits at three time points, 30 days gestation and 4 and 17 weeks postnatal age. Morphometric measures (mean caliper diameter, surface area, and volume) of nonciliated and ciliated bronchiolar cell nuclei, using computer modeling from serial sections, showed a significant decrease in nuclear size for both cell types and a significant increase in cell volume for the nonciliated bronchiolar cell during lung maturation. A shape coefficient (β) proved to be the most efficient estimator of the number of cells per unit volume when it was used with estimates of the number of nuclei per unit area and the volumetric density of nuclei. Two-dimensional estimates of bronchiolar epithelial cell abundance (the number of nuclei per unit length or area) significantly underestimated the percentage of nonciliated bronchiolar cells as compared to three-dimensional estimates for rabbits 17 weeks of age. We have shown an inverse relationship between nonciliated and ciliated bronchiolar cell abundance during lung maturation. Nonciliated cells decreased while ciliated cells increased. We have confirmed that cytodifferentiation of the nonciliated bronchiolar cell occurs within the first 4 weeks of postnatal development. The volume of the nonciliated bronchiolar cell increased about twofold during development. Because of the concomitant decrease in nuclear volume, the cytoplasm o the cell showed an even. Within the cytoplasm of the nonciliated bronchiolar cell, glycogen significantly decreased, and agranular endoplasmic reticulum (AER) and mitochondria significantly increased in volume during development. The biosynthesis of AER closely correlated with pharmacological studies of xenobiotic metabolism during rabbit lung maturation.
UR - http://www.scopus.com/inward/record.url?scp=0020617044&partnerID=8YFLogxK
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M3 - Article
C2 - 6881073
AN - SCOPUS:0020617044
VL - 167
SP - 359
EP - 370
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 3
ER -