Abstract
Schwann cell cultures are difficult to obtain from adult rat because of the abundant amount of connective tissue and myelin. We have developed a method for isolation and culture of these cells by mechanical and chemical dissociation which represents a modification of a previously described procedure for human neurofibromas. Schwann cells were identified in indirect immunofluorescence by the capacity to bind antibodies to S-100, galactocerebroside, and laminin. The baseline cell proliferation index was assessed by immunofluorescence of bromodeoxyuridine. This method provides Schwann cells from adult rat nerves for at least 7 days and in sufficient numbers for (a) morphological and immunological characterization and (b) analysis of the effects of mitogenic factors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 167-176 |
| Number of pages | 10 |
| Journal | Experimental Neurology |
| Volume | 102 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1988 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
- Neurology
Cite this
Establishment of Schwann cell cultures from adult rat peripheral nerves. / Scarpini, Elio; Kreider, Barbara Q.; Lisak, Robert P.; Pleasure, David E.
In: Experimental Neurology, Vol. 102, No. 2, 1988, p. 167-176.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Establishment of Schwann cell cultures from adult rat peripheral nerves
AU - Scarpini, Elio
AU - Kreider, Barbara Q.
AU - Lisak, Robert P.
AU - Pleasure, David E
PY - 1988
Y1 - 1988
N2 - Schwann cell cultures are difficult to obtain from adult rat because of the abundant amount of connective tissue and myelin. We have developed a method for isolation and culture of these cells by mechanical and chemical dissociation which represents a modification of a previously described procedure for human neurofibromas. Schwann cells were identified in indirect immunofluorescence by the capacity to bind antibodies to S-100, galactocerebroside, and laminin. The baseline cell proliferation index was assessed by immunofluorescence of bromodeoxyuridine. This method provides Schwann cells from adult rat nerves for at least 7 days and in sufficient numbers for (a) morphological and immunological characterization and (b) analysis of the effects of mitogenic factors.
AB - Schwann cell cultures are difficult to obtain from adult rat because of the abundant amount of connective tissue and myelin. We have developed a method for isolation and culture of these cells by mechanical and chemical dissociation which represents a modification of a previously described procedure for human neurofibromas. Schwann cells were identified in indirect immunofluorescence by the capacity to bind antibodies to S-100, galactocerebroside, and laminin. The baseline cell proliferation index was assessed by immunofluorescence of bromodeoxyuridine. This method provides Schwann cells from adult rat nerves for at least 7 days and in sufficient numbers for (a) morphological and immunological characterization and (b) analysis of the effects of mitogenic factors.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023781619&partnerID=8YFLogxK
U2 - 10.1016/0014-4886(88)90090-8
DO - 10.1016/0014-4886(88)90090-8
M3 - Article
VL - 102
SP - 167
EP - 176
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 2
ER -