Establishment of Schwann cell cultures from adult rat peripheral nerves

Elio Scarpini, Barbara Q. Kreider, Robert P. Lisak, David E Pleasure

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Schwann cell cultures are difficult to obtain from adult rat because of the abundant amount of connective tissue and myelin. We have developed a method for isolation and culture of these cells by mechanical and chemical dissociation which represents a modification of a previously described procedure for human neurofibromas. Schwann cells were identified in indirect immunofluorescence by the capacity to bind antibodies to S-100, galactocerebroside, and laminin. The baseline cell proliferation index was assessed by immunofluorescence of bromodeoxyuridine. This method provides Schwann cells from adult rat nerves for at least 7 days and in sufficient numbers for (a) morphological and immunological characterization and (b) analysis of the effects of mitogenic factors.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalExperimental Neurology
Volume102
Issue number2
DOIs
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

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