Establishment of Schwann cell cultures from adult rat peripheral nerves

Elio Scarpini, Barbara Q. Kreider, Robert P. Lisak, David E Pleasure

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Schwann cell cultures are difficult to obtain from adult rat because of the abundant amount of connective tissue and myelin. We have developed a method for isolation and culture of these cells by mechanical and chemical dissociation which represents a modification of a previously described procedure for human neurofibromas. Schwann cells were identified in indirect immunofluorescence by the capacity to bind antibodies to S-100, galactocerebroside, and laminin. The baseline cell proliferation index was assessed by immunofluorescence of bromodeoxyuridine. This method provides Schwann cells from adult rat nerves for at least 7 days and in sufficient numbers for (a) morphological and immunological characterization and (b) analysis of the effects of mitogenic factors.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalExperimental Neurology
Volume102
Issue number2
DOIs
StatePublished - 1988
Externally publishedYes

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Schwann Cells
Peripheral Nerves
Cell Culture Techniques
Neurofibroma
Cell Separation
Laminin
Bromodeoxyuridine
Indirect Fluorescent Antibody Technique
Myelin Sheath
Connective Tissue
Fluorescent Antibody Technique
Cell Proliferation
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neurology

Cite this

Establishment of Schwann cell cultures from adult rat peripheral nerves. / Scarpini, Elio; Kreider, Barbara Q.; Lisak, Robert P.; Pleasure, David E.

In: Experimental Neurology, Vol. 102, No. 2, 1988, p. 167-176.

Research output: Contribution to journalArticle

Scarpini, Elio ; Kreider, Barbara Q. ; Lisak, Robert P. ; Pleasure, David E. / Establishment of Schwann cell cultures from adult rat peripheral nerves. In: Experimental Neurology. 1988 ; Vol. 102, No. 2. pp. 167-176.
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