Abstract
Utilizing human recombinant interleukin 2 (HrIL-2) in combination with several mitogens, continuously growing cultures of bovine lymphocytes have been established. Continuous presence of HrIL-2 is required to maintain the growth of these cultures but a requirement for continued presence of mitogen seems variable. Cloned lines have been derived from these cultures by limiting dilution and outgrowth in the presence of mitomvcin C treated bulk cells. Cell surface phenotype analysis indicates that all of these cultures and cloned lines are of the T cell lineage. Functional analysis indicated that some of these cultured cells are cytolytically active in lectin mediated assays whereas others are not. Utilization of this technology for the growth and characterization of bovine T cells will undoubtedly contribute to an enhanced understanding of the bovine immune response mechanisms.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 209-222 |
| Number of pages | 14 |
| Journal | Veterinary Immunology and Immunopathology |
| Volume | 14 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Animal Science and Zoology
- Immunology
- veterinary(all)
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Establishment and initial characterization of continuous in vitro cultures of bovine T lymphocytes. / Scibienski, R. J.; Stott, M. L.; Stott, Jeffrey L.
In: Veterinary Immunology and Immunopathology, Vol. 14, No. 3, 1987, p. 209-222.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Establishment and initial characterization of continuous in vitro cultures of bovine T lymphocytes
AU - Scibienski, R. J.
AU - Stott, M. L.
AU - Stott, Jeffrey L
PY - 1987
Y1 - 1987
N2 - Utilizing human recombinant interleukin 2 (HrIL-2) in combination with several mitogens, continuously growing cultures of bovine lymphocytes have been established. Continuous presence of HrIL-2 is required to maintain the growth of these cultures but a requirement for continued presence of mitogen seems variable. Cloned lines have been derived from these cultures by limiting dilution and outgrowth in the presence of mitomvcin C treated bulk cells. Cell surface phenotype analysis indicates that all of these cultures and cloned lines are of the T cell lineage. Functional analysis indicated that some of these cultured cells are cytolytically active in lectin mediated assays whereas others are not. Utilization of this technology for the growth and characterization of bovine T cells will undoubtedly contribute to an enhanced understanding of the bovine immune response mechanisms.
AB - Utilizing human recombinant interleukin 2 (HrIL-2) in combination with several mitogens, continuously growing cultures of bovine lymphocytes have been established. Continuous presence of HrIL-2 is required to maintain the growth of these cultures but a requirement for continued presence of mitogen seems variable. Cloned lines have been derived from these cultures by limiting dilution and outgrowth in the presence of mitomvcin C treated bulk cells. Cell surface phenotype analysis indicates that all of these cultures and cloned lines are of the T cell lineage. Functional analysis indicated that some of these cultured cells are cytolytically active in lectin mediated assays whereas others are not. Utilization of this technology for the growth and characterization of bovine T cells will undoubtedly contribute to an enhanced understanding of the bovine immune response mechanisms.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023094066&partnerID=8YFLogxK
U2 - 10.1016/0165-2427(87)90090-0
DO - 10.1016/0165-2427(87)90090-0
M3 - Article
C2 - 3109112
AN - SCOPUS:0023094066
VL - 14
SP - 209
EP - 222
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 3
ER -