Establishment and characterization of baboon embryonic stem cell lines: An Old World Primate model for regeneration and transplantation research

Calvin R. Simerly, Christopher S. Navara, Carlos A. Castro, Janet C. Turpin, Carrie J. Redinger, Jocelyn D. Mich-Basso, Ethan S. Jacoby, Kevin J. Grund, David A. McFarland, Stacie L. Oliver, Ahmi Ben-Yehudah, Diane L. Carlisle, Patricia Frost, Cecilia Penedo, Laura Hewitson, Gerald Schatten

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Here we have developed protocols using the baboon as a complementary alternative Old World Primate to rhesus and other macaques which have severe limitations in their availability. Baboons are not limited as research resources, they are evolutionarily closer to humans, and the multiple generations of pedigreed colonies which display complex human disease phenotypes all support their further optimization as an invaluable primate model. Since neither baboon-assisted reproductive technologies nor baboon embryonic stem cells (ESCs) have been reported, here we describe the first derivations and characterization of baboon ESC lines from IVF-generated blastocysts. Two ESCs lines (BabESC-4 and BabESC-15) display ESC morphology, express pluripotency markers (Oct-4, hTert, Nanog, Sox-2, Rex-1, TRA1-60, TRA1-81), and maintain stable euploid female karyotypes with parentage confirmed independently. They have been grown continuously for > 430 and 290 days, respectively. Teratomas from both lines have all three germ layers. Availabilities of these BabESCs represent another important resource for stem cell biologists.

Original languageEnglish (US)
Pages (from-to)178-187
Number of pages10
JournalStem Cell Research
Volume2
Issue number3
DOIs
StatePublished - May 1 2009

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Papio
Embryonic Stem Cells
Primates
Regeneration
Transplantation
Cell Line
Research
Germ Layers
Assisted Reproductive Techniques
Teratoma
Blastocyst
Macaca mulatta
Karyotype
Stem Cells
Phenotype

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Simerly, C. R., Navara, C. S., Castro, C. A., Turpin, J. C., Redinger, C. J., Mich-Basso, J. D., ... Schatten, G. (2009). Establishment and characterization of baboon embryonic stem cell lines: An Old World Primate model for regeneration and transplantation research. Stem Cell Research, 2(3), 178-187. https://doi.org/10.1016/j.scr.2009.02.004

Establishment and characterization of baboon embryonic stem cell lines : An Old World Primate model for regeneration and transplantation research. / Simerly, Calvin R.; Navara, Christopher S.; Castro, Carlos A.; Turpin, Janet C.; Redinger, Carrie J.; Mich-Basso, Jocelyn D.; Jacoby, Ethan S.; Grund, Kevin J.; McFarland, David A.; Oliver, Stacie L.; Ben-Yehudah, Ahmi; Carlisle, Diane L.; Frost, Patricia; Penedo, Cecilia; Hewitson, Laura; Schatten, Gerald.

In: Stem Cell Research, Vol. 2, No. 3, 01.05.2009, p. 178-187.

Research output: Contribution to journalArticle

Simerly, CR, Navara, CS, Castro, CA, Turpin, JC, Redinger, CJ, Mich-Basso, JD, Jacoby, ES, Grund, KJ, McFarland, DA, Oliver, SL, Ben-Yehudah, A, Carlisle, DL, Frost, P, Penedo, C, Hewitson, L & Schatten, G 2009, 'Establishment and characterization of baboon embryonic stem cell lines: An Old World Primate model for regeneration and transplantation research', Stem Cell Research, vol. 2, no. 3, pp. 178-187. https://doi.org/10.1016/j.scr.2009.02.004
Simerly, Calvin R. ; Navara, Christopher S. ; Castro, Carlos A. ; Turpin, Janet C. ; Redinger, Carrie J. ; Mich-Basso, Jocelyn D. ; Jacoby, Ethan S. ; Grund, Kevin J. ; McFarland, David A. ; Oliver, Stacie L. ; Ben-Yehudah, Ahmi ; Carlisle, Diane L. ; Frost, Patricia ; Penedo, Cecilia ; Hewitson, Laura ; Schatten, Gerald. / Establishment and characterization of baboon embryonic stem cell lines : An Old World Primate model for regeneration and transplantation research. In: Stem Cell Research. 2009 ; Vol. 2, No. 3. pp. 178-187.
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