Abstract
Here we have developed protocols using the baboon as a complementary alternative Old World Primate to rhesus and other macaques which have severe limitations in their availability. Baboons are not limited as research resources, they are evolutionarily closer to humans, and the multiple generations of pedigreed colonies which display complex human disease phenotypes all support their further optimization as an invaluable primate model. Since neither baboon-assisted reproductive technologies nor baboon embryonic stem cells (ESCs) have been reported, here we describe the first derivations and characterization of baboon ESC lines from IVF-generated blastocysts. Two ESCs lines (BabESC-4 and BabESC-15) display ESC morphology, express pluripotency markers (Oct-4, hTert, Nanog, Sox-2, Rex-1, TRA1-60, TRA1-81), and maintain stable euploid female karyotypes with parentage confirmed independently. They have been grown continuously for > 430 and 290 days, respectively. Teratomas from both lines have all three germ layers. Availabilities of these BabESCs represent another important resource for stem cell biologists.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 178-187 |
| Number of pages | 10 |
| Journal | Stem Cell Research |
| Volume | 2 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1 2009 |
Fingerprint
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Establishment and characterization of baboon embryonic stem cell lines : An Old World Primate model for regeneration and transplantation research. / Simerly, Calvin R.; Navara, Christopher S.; Castro, Carlos A.; Turpin, Janet C.; Redinger, Carrie J.; Mich-Basso, Jocelyn D.; Jacoby, Ethan S.; Grund, Kevin J.; McFarland, David A.; Oliver, Stacie L.; Ben-Yehudah, Ahmi; Carlisle, Diane L.; Frost, Patricia; Penedo, Cecilia; Hewitson, Laura; Schatten, Gerald.
In: Stem Cell Research, Vol. 2, No. 3, 01.05.2009, p. 178-187.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Establishment and characterization of baboon embryonic stem cell lines
T2 - An Old World Primate model for regeneration and transplantation research
AU - Simerly, Calvin R.
AU - Navara, Christopher S.
AU - Castro, Carlos A.
AU - Turpin, Janet C.
AU - Redinger, Carrie J.
AU - Mich-Basso, Jocelyn D.
AU - Jacoby, Ethan S.
AU - Grund, Kevin J.
AU - McFarland, David A.
AU - Oliver, Stacie L.
AU - Ben-Yehudah, Ahmi
AU - Carlisle, Diane L.
AU - Frost, Patricia
AU - Penedo, Cecilia
AU - Hewitson, Laura
AU - Schatten, Gerald
PY - 2009/5/1
Y1 - 2009/5/1
N2 - Here we have developed protocols using the baboon as a complementary alternative Old World Primate to rhesus and other macaques which have severe limitations in their availability. Baboons are not limited as research resources, they are evolutionarily closer to humans, and the multiple generations of pedigreed colonies which display complex human disease phenotypes all support their further optimization as an invaluable primate model. Since neither baboon-assisted reproductive technologies nor baboon embryonic stem cells (ESCs) have been reported, here we describe the first derivations and characterization of baboon ESC lines from IVF-generated blastocysts. Two ESCs lines (BabESC-4 and BabESC-15) display ESC morphology, express pluripotency markers (Oct-4, hTert, Nanog, Sox-2, Rex-1, TRA1-60, TRA1-81), and maintain stable euploid female karyotypes with parentage confirmed independently. They have been grown continuously for > 430 and 290 days, respectively. Teratomas from both lines have all three germ layers. Availabilities of these BabESCs represent another important resource for stem cell biologists.
AB - Here we have developed protocols using the baboon as a complementary alternative Old World Primate to rhesus and other macaques which have severe limitations in their availability. Baboons are not limited as research resources, they are evolutionarily closer to humans, and the multiple generations of pedigreed colonies which display complex human disease phenotypes all support their further optimization as an invaluable primate model. Since neither baboon-assisted reproductive technologies nor baboon embryonic stem cells (ESCs) have been reported, here we describe the first derivations and characterization of baboon ESC lines from IVF-generated blastocysts. Two ESCs lines (BabESC-4 and BabESC-15) display ESC morphology, express pluripotency markers (Oct-4, hTert, Nanog, Sox-2, Rex-1, TRA1-60, TRA1-81), and maintain stable euploid female karyotypes with parentage confirmed independently. They have been grown continuously for > 430 and 290 days, respectively. Teratomas from both lines have all three germ layers. Availabilities of these BabESCs represent another important resource for stem cell biologists.
UR - http://www.scopus.com/inward/record.url?scp=67349281279&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349281279&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2009.02.004
DO - 10.1016/j.scr.2009.02.004
M3 - Article
C2 - 19393591
AN - SCOPUS:67349281279
VL - 2
SP - 178
EP - 187
JO - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
IS - 3
ER -