Escherichia coli persistence kinetics in dairy manure at moderate, mesophilic, and thermophilic temperatures under aerobic and anaerobic environments

Pramod Pandey, Sagor Biswas, Venkata K. Vaddella, Michelle L. Soupir

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To assess Escherichia coli (E. coli) persistence in dairy manure, bench scale experiments were conducted under aerobic and anaerobic environments. The changes in E. coli levels in dairy manure were assessed at moderate (25 °C), mesophilic (37 °C), and thermophilic (52.5 °C) temperatures. The inactivation of E. coli at moderate, mesophilic, and thermophilic temperatures were described by linear regression equations. Subsequently, double-exponential kinetic models were developed to describe the E. coli decay curves under aerobic and anaerobic environments. The kinetics models were used to estimate E. coli log reductions at various temperatures. Results showed that the double-exponential kinetic models performed well while calculating E. coli reductions in dairy manure over the incubation period. In addition, we evaluated digestate to compare the changes in total solids and volatile solids, total organic carbon, total nitrogen, pH, and oxygen reduction potential levels in aerobic and anaerobic conditions under various temperatures. We anticipate that the results presented here will be useful for enhancing the understanding of pathogen reduction in anaerobic and aerobic processes during dairy manure treatment.

Original languageEnglish (US)
Pages (from-to)457-467
Number of pages11
JournalBioprocess and Biosystems Engineering
Volume38
Issue number3
DOIs
StatePublished - Feb 26 2015

Fingerprint

Manure
Dairies
Manures
Escherichia coli
Kinetics
Temperature
Pathogens
Organic carbon
Linear regression
Linear Models
Nitrogen
Carbon
Oxygen
Experiments

Keywords

  • Aerobic
  • Anaerobic
  • Dairy manure
  • E. coli
  • Inactivation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Escherichia coli persistence kinetics in dairy manure at moderate, mesophilic, and thermophilic temperatures under aerobic and anaerobic environments. / Pandey, Pramod; Biswas, Sagor; Vaddella, Venkata K.; Soupir, Michelle L.

In: Bioprocess and Biosystems Engineering, Vol. 38, No. 3, 26.02.2015, p. 457-467.

Research output: Contribution to journalArticle

@article{6523332e5e1e41d8a385339e713d7222,
title = "Escherichia coli persistence kinetics in dairy manure at moderate, mesophilic, and thermophilic temperatures under aerobic and anaerobic environments",
abstract = "To assess Escherichia coli (E. coli) persistence in dairy manure, bench scale experiments were conducted under aerobic and anaerobic environments. The changes in E. coli levels in dairy manure were assessed at moderate (25 °C), mesophilic (37 °C), and thermophilic (52.5 °C) temperatures. The inactivation of E. coli at moderate, mesophilic, and thermophilic temperatures were described by linear regression equations. Subsequently, double-exponential kinetic models were developed to describe the E. coli decay curves under aerobic and anaerobic environments. The kinetics models were used to estimate E. coli log reductions at various temperatures. Results showed that the double-exponential kinetic models performed well while calculating E. coli reductions in dairy manure over the incubation period. In addition, we evaluated digestate to compare the changes in total solids and volatile solids, total organic carbon, total nitrogen, pH, and oxygen reduction potential levels in aerobic and anaerobic conditions under various temperatures. We anticipate that the results presented here will be useful for enhancing the understanding of pathogen reduction in anaerobic and aerobic processes during dairy manure treatment.",
keywords = "Aerobic, Anaerobic, Dairy manure, E. coli, Inactivation",
author = "Pramod Pandey and Sagor Biswas and Vaddella, {Venkata K.} and Soupir, {Michelle L.}",
year = "2015",
month = "2",
day = "26",
doi = "10.1007/s00449-014-1285-3",
language = "English (US)",
volume = "38",
pages = "457--467",
journal = "Bioprocess and Biosystems Engineering",
issn = "1615-7591",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Escherichia coli persistence kinetics in dairy manure at moderate, mesophilic, and thermophilic temperatures under aerobic and anaerobic environments

AU - Pandey, Pramod

AU - Biswas, Sagor

AU - Vaddella, Venkata K.

AU - Soupir, Michelle L.

PY - 2015/2/26

Y1 - 2015/2/26

N2 - To assess Escherichia coli (E. coli) persistence in dairy manure, bench scale experiments were conducted under aerobic and anaerobic environments. The changes in E. coli levels in dairy manure were assessed at moderate (25 °C), mesophilic (37 °C), and thermophilic (52.5 °C) temperatures. The inactivation of E. coli at moderate, mesophilic, and thermophilic temperatures were described by linear regression equations. Subsequently, double-exponential kinetic models were developed to describe the E. coli decay curves under aerobic and anaerobic environments. The kinetics models were used to estimate E. coli log reductions at various temperatures. Results showed that the double-exponential kinetic models performed well while calculating E. coli reductions in dairy manure over the incubation period. In addition, we evaluated digestate to compare the changes in total solids and volatile solids, total organic carbon, total nitrogen, pH, and oxygen reduction potential levels in aerobic and anaerobic conditions under various temperatures. We anticipate that the results presented here will be useful for enhancing the understanding of pathogen reduction in anaerobic and aerobic processes during dairy manure treatment.

AB - To assess Escherichia coli (E. coli) persistence in dairy manure, bench scale experiments were conducted under aerobic and anaerobic environments. The changes in E. coli levels in dairy manure were assessed at moderate (25 °C), mesophilic (37 °C), and thermophilic (52.5 °C) temperatures. The inactivation of E. coli at moderate, mesophilic, and thermophilic temperatures were described by linear regression equations. Subsequently, double-exponential kinetic models were developed to describe the E. coli decay curves under aerobic and anaerobic environments. The kinetics models were used to estimate E. coli log reductions at various temperatures. Results showed that the double-exponential kinetic models performed well while calculating E. coli reductions in dairy manure over the incubation period. In addition, we evaluated digestate to compare the changes in total solids and volatile solids, total organic carbon, total nitrogen, pH, and oxygen reduction potential levels in aerobic and anaerobic conditions under various temperatures. We anticipate that the results presented here will be useful for enhancing the understanding of pathogen reduction in anaerobic and aerobic processes during dairy manure treatment.

KW - Aerobic

KW - Anaerobic

KW - Dairy manure

KW - E. coli

KW - Inactivation

UR - http://www.scopus.com/inward/record.url?scp=84938060332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938060332&partnerID=8YFLogxK

U2 - 10.1007/s00449-014-1285-3

DO - 10.1007/s00449-014-1285-3

M3 - Article

C2 - 25248871

AN - SCOPUS:84938060332

VL - 38

SP - 457

EP - 467

JO - Bioprocess and Biosystems Engineering

JF - Bioprocess and Biosystems Engineering

SN - 1615-7591

IS - 3

ER -