Erythrocyte cation-activated adenosine triphosphatases in duchenne muscular dystrophy

Andrew Hodson, David E Pleasure

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The cation-stimulated adenosine triphosphatase (ATPase) activities of erythrocyte ghosts and erythrocyte ghost plasma membrane fragments of patients with Duchenne muscular dystrophy (DMD) were compared with activities in age-matched normal male controls. DMD Mg++-stimulated ATPase activity was within the normal range. The specific activity of DMD erythrocyte ghost Na+, K+-stimulated, Mg++-dependent ATPase was also normal, and was inhibited by 10-4 M ouabain to an extent comparable with controls. Ca++-stimulated, Mg++-dependent ATPase activity of DMD erythrocyte ghost plasma membrane fragments, assayed at 0.5 mM free Ca++, was 21% above that in age-matched male controls (n = 22, 2-tailed paired t-test, P < 0.01). Kinetic studies indicated that the DMD erythrocyte Ca++-stimulated, Mg++-dependent ATPase has greater affinity for MgATP than the enzyme in control erythrocytes.

Original languageEnglish (US)
Pages (from-to)361-369
Number of pages9
JournalJournal of the Neurological Sciences
Volume32
Issue number3
DOIs
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

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