ERCC2

CDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3

Christine A. Weber, Edmund P. Salazar, Sheri A. Stewart, Larry H. Thompson

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

Human ERCC2 genomic clones give efficient, stable correction of the nucleotide excision repair defect in UV5 Chinese hamster ovary cells. One clone having a breakpoint just 5′ of classical promoter elements corrects only transiently, implicating further flanking sequences in stable gene expression. The nucleotide sequences of a cDNA clone and genomic flanking regions were determined. The ERCC2 translated amino acid sequence has 52% identity (73% homology) with the yeast nucleotide excision repair protein RAD3. RAD3 is essential for cell viability and encodes a protein that is a single-stranded DNA dependent ATPase and an ATP dependent helicase. The similarity of ERCC2 and RAD3 suggests a role for ERCC2 in both cell viability and DNA repair and provides the first insight into the biochemical function of a mammalian nucleotide excision repair gene.

Original languageEnglish (US)
Pages (from-to)1437-1447
Number of pages11
JournalEMBO Journal
Volume9
Issue number5
StatePublished - 1990
Externally publishedYes

Fingerprint

Cloning
Molecular Cloning
DNA Repair
Yeast
Repair
Nucleotides
Genes
Yeasts
Clone Cells
Cells
Cell Survival
Cricetulus
Gene expression
Amino Acid Sequence
Ovary
Proteins
Complementary DNA
Adenosine Triphosphate
Gene Expression
Amino Acids

Keywords

  • Chromosome 19
  • DNA repair
  • Gene cloning
  • Nucleotide excision repair

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Weber, C. A., Salazar, E. P., Stewart, S. A., & Thompson, L. H. (1990). ERCC2: CDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3. EMBO Journal, 9(5), 1437-1447.

ERCC2 : CDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3. / Weber, Christine A.; Salazar, Edmund P.; Stewart, Sheri A.; Thompson, Larry H.

In: EMBO Journal, Vol. 9, No. 5, 1990, p. 1437-1447.

Research output: Contribution to journalArticle

Weber, CA, Salazar, EP, Stewart, SA & Thompson, LH 1990, 'ERCC2: CDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3', EMBO Journal, vol. 9, no. 5, pp. 1437-1447.
Weber, Christine A. ; Salazar, Edmund P. ; Stewart, Sheri A. ; Thompson, Larry H. / ERCC2 : CDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3. In: EMBO Journal. 1990 ; Vol. 9, No. 5. pp. 1437-1447.
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