ERCC2: CDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3

Christine A. Weber, Edmund P. Salazar, Sheri A. Stewart, Larry H. Thompson

Research output: Contribution to journalArticle

234 Scopus citations

Abstract

Human ERCC2 genomic clones give efficient, stable correction of the nucleotide excision repair defect in UV5 Chinese hamster ovary cells. One clone having a breakpoint just 5′ of classical promoter elements corrects only transiently, implicating further flanking sequences in stable gene expression. The nucleotide sequences of a cDNA clone and genomic flanking regions were determined. The ERCC2 translated amino acid sequence has 52% identity (73% homology) with the yeast nucleotide excision repair protein RAD3. RAD3 is essential for cell viability and encodes a protein that is a single-stranded DNA dependent ATPase and an ATP dependent helicase. The similarity of ERCC2 and RAD3 suggests a role for ERCC2 in both cell viability and DNA repair and provides the first insight into the biochemical function of a mammalian nucleotide excision repair gene.

Original languageEnglish (US)
Pages (from-to)1437-1447
Number of pages11
JournalEMBO Journal
Volume9
Issue number5
StatePublished - 1990
Externally publishedYes

Keywords

  • Chromosome 19
  • DNA repair
  • Gene cloning
  • Nucleotide excision repair

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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    Weber, C. A., Salazar, E. P., Stewart, S. A., & Thompson, L. H. (1990). ERCC2: CDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3. EMBO Journal, 9(5), 1437-1447.