ER-mitochondria contacts couple mtDNA synthesis with Mitochondrial division in human cells

Samantha C. Lewis, Lauren F. Uchiyama, Jodi Nunnari

Research output: Contribution to journalArticlepeer-review

276 Scopus citations


Mitochondrial DNA (mtDNA) encodes RNAs and proteins critical for cell function. In human cells, hundreds to thousands of mtDNA copies are replicated asynchronously, packaged into protein-DNA nucleoids, and distributed within a dynamic mitochondrial network. The mechanisms that govern how nucleoids are chosen for replication and distribution are not understood. Mitochondrial distribution depends on division, which occurs at endoplasmic reticulum (ER)-mitochondria contact sites. These sites were spatially linked to a subset of nucleoids selectively marked by mtDNA polymerase and engaged in mtDNA synthesis-events that occurred upstream of mitochondrial constriction and division machine assembly. Our data suggest that ER tubules proximal to nucleoids are necessary but not sufficient for mtDNA synthesis. Thus, ER-mitochondria contacts coordinate licensing of mtDNA synthesis with division to distribute newly replicated nucleoids to daughter mitochondria.

Original languageEnglish (US)
Article numberaaf5549
Issue number6296
StatePublished - Jul 15 2016

ASJC Scopus subject areas

  • Medicine(all)
  • General


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