Equine synteny mapping of comparative anchor tagged sequences (CATS) from human Chromosome 5

Alexandre R. Caetano, Leslie A Lyons, Thomas F. Laughlin, Stephen J. O'Brien, James D. Murray, Ann T. Bowling

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Comparative anchor tagged sequences (CATS) from human Chromosome 5 (HSA5) were used as PCR primers to produce molecular markers for synteny mapping in the horse. Primer sets for 21 genes yielded eight horse-specific markers, which were mapped with the UC Davis horse-mouse somatic cell hybrid panel into two synteny groups: UCD14 and UCD21. These data, in conjunction with earlier human chromosome painting studies of the horse karyotype and synteny mapping of horse microsatellite markers physically mapped by FISH, confirm the assignment of UCD21 to ECA21 and suggest that UCD14 is located on ECA14. In addition, our results can be used to substantiate previously published data which indicate that ECA21 contains material orthologous to HSA5p and HSA5q, and to propose an approximate region for an evolutionary chromosomal rearrangement event.

Original languageEnglish (US)
Pages (from-to)1082-1084
Number of pages3
JournalMammalian Genome
Volume10
Issue number11
DOIs
StatePublished - 1999
Externally publishedYes

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Synteny
Chromosomes, Human, Pair 5
Human Chromosomes
Horses
Chromosome Painting
Hybrid Cells
Karyotype
Microsatellite Repeats
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Caetano, A. R., Lyons, L. A., Laughlin, T. F., O'Brien, S. J., Murray, J. D., & Bowling, A. T. (1999). Equine synteny mapping of comparative anchor tagged sequences (CATS) from human Chromosome 5. Mammalian Genome, 10(11), 1082-1084. https://doi.org/10.1007/s003359901165

Equine synteny mapping of comparative anchor tagged sequences (CATS) from human Chromosome 5. / Caetano, Alexandre R.; Lyons, Leslie A; Laughlin, Thomas F.; O'Brien, Stephen J.; Murray, James D.; Bowling, Ann T.

In: Mammalian Genome, Vol. 10, No. 11, 1999, p. 1082-1084.

Research output: Contribution to journalArticle

Caetano, AR, Lyons, LA, Laughlin, TF, O'Brien, SJ, Murray, JD & Bowling, AT 1999, 'Equine synteny mapping of comparative anchor tagged sequences (CATS) from human Chromosome 5', Mammalian Genome, vol. 10, no. 11, pp. 1082-1084. https://doi.org/10.1007/s003359901165
Caetano, Alexandre R. ; Lyons, Leslie A ; Laughlin, Thomas F. ; O'Brien, Stephen J. ; Murray, James D. ; Bowling, Ann T. / Equine synteny mapping of comparative anchor tagged sequences (CATS) from human Chromosome 5. In: Mammalian Genome. 1999 ; Vol. 10, No. 11. pp. 1082-1084.
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