Abstract
Comparative anchor tagged sequences (CATS) from human Chromosome 5 (HSA5) were used as PCR primers to produce molecular markers for synteny mapping in the horse. Primer sets for 21 genes yielded eight horse-specific markers, which were mapped with the UC Davis horse-mouse somatic cell hybrid panel into two synteny groups: UCD14 and UCD21. These data, in conjunction with earlier human chromosome painting studies of the horse karyotype and synteny mapping of horse microsatellite markers physically mapped by FISH, confirm the assignment of UCD21 to ECA21 and suggest that UCD14 is located on ECA14. In addition, our results can be used to substantiate previously published data which indicate that ECA21 contains material orthologous to HSA5p and HSA5q, and to propose an approximate region for an evolutionary chromosomal rearrangement event.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1082-1084 |
| Number of pages | 3 |
| Journal | Mammalian Genome |
| Volume | 10 |
| Issue number | 11 |
| DOIs | |
| State | Published - 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
Equine synteny mapping of comparative anchor tagged sequences (CATS) from human Chromosome 5. / Caetano, Alexandre R.; Lyons, Leslie A; Laughlin, Thomas F.; O'Brien, Stephen J.; Murray, James D.; Bowling, Ann T.
In: Mammalian Genome, Vol. 10, No. 11, 1999, p. 1082-1084.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Equine synteny mapping of comparative anchor tagged sequences (CATS) from human Chromosome 5
AU - Caetano, Alexandre R.
AU - Lyons, Leslie A
AU - Laughlin, Thomas F.
AU - O'Brien, Stephen J.
AU - Murray, James D.
AU - Bowling, Ann T.
PY - 1999
Y1 - 1999
N2 - Comparative anchor tagged sequences (CATS) from human Chromosome 5 (HSA5) were used as PCR primers to produce molecular markers for synteny mapping in the horse. Primer sets for 21 genes yielded eight horse-specific markers, which were mapped with the UC Davis horse-mouse somatic cell hybrid panel into two synteny groups: UCD14 and UCD21. These data, in conjunction with earlier human chromosome painting studies of the horse karyotype and synteny mapping of horse microsatellite markers physically mapped by FISH, confirm the assignment of UCD21 to ECA21 and suggest that UCD14 is located on ECA14. In addition, our results can be used to substantiate previously published data which indicate that ECA21 contains material orthologous to HSA5p and HSA5q, and to propose an approximate region for an evolutionary chromosomal rearrangement event.
AB - Comparative anchor tagged sequences (CATS) from human Chromosome 5 (HSA5) were used as PCR primers to produce molecular markers for synteny mapping in the horse. Primer sets for 21 genes yielded eight horse-specific markers, which were mapped with the UC Davis horse-mouse somatic cell hybrid panel into two synteny groups: UCD14 and UCD21. These data, in conjunction with earlier human chromosome painting studies of the horse karyotype and synteny mapping of horse microsatellite markers physically mapped by FISH, confirm the assignment of UCD21 to ECA21 and suggest that UCD14 is located on ECA14. In addition, our results can be used to substantiate previously published data which indicate that ECA21 contains material orthologous to HSA5p and HSA5q, and to propose an approximate region for an evolutionary chromosomal rearrangement event.
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U2 - 10.1007/s003359901165
DO - 10.1007/s003359901165
M3 - Article
C2 - 10556427
AN - SCOPUS:0032740977
VL - 10
SP - 1082
EP - 1084
JO - Mammalian Genome
JF - Mammalian Genome
SN - 0938-8990
IS - 11
ER -