Equine sperm-oocyte interaction: The role of sperm surface hyaluronidase

Research output: Contribution to journalArticle

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Abstract

The plasma membrane over the sperm head of several mammalian species has been shown to express a glycerolphosphatidylinositol-linked hyaluronidase known as PH-20. This protein has been associated with the sperm's interaction with the oocyte cumulus matrix and zona pellucida. The characteristics of PH-20 in equine sperm have not been clearly defined. In this study, ejaculated gel-free semen from five stallions and epididymal sperm from isolated epididymis from 10 stallions was used to characterize the PH-20 activity in equine sperm. Affinity purified anti-equine PH-20 polyclonal antibody was used to immunodetect sperm surface-associated PH-20 and immunolabel whole sperm. The intracellular calcium indicator, Fluo-3, was used to assess sperm intracellular calcium. Stallion sperm express a surface-associated hyaluronidase localized to the posterior sperm head region in ejaculated sperm. Following in vitro capacitation and acrosomal exocytosis, the inner acrosomal membrane (lAM) displays intense hyaluronidase fluorescence suggesting that the IAM and hyaluronidase plays a significant role in zona penetration by sperm. Sperm incubated in hyaluronan (HA)-containing capacitation medium display an elevated intracellular calcium concentration (P < 0.01) that is associated with translocation of PH-20 antigenic sites on the sperm surface in addition to increases in protein tyrosine phosphorylation. Caput- and cauda-derived sperm display developmentally unique PH-20 immunofluorescence expression patterns. These data suggest that the differential expression of PH-20 in ejaculated and epididymal sperm could be involved in cumulus penetration, sperm-egg recognition, and oolemmal fusion in this species.

Original languageEnglish (US)
Pages (from-to)291-303
Number of pages13
JournalAnimal Reproduction Science
Volume68
Issue number3-4
DOIs
StatePublished - Dec 3 2001

Fingerprint

Sperm-Ovum Interactions
hyaluronoglucosaminidase
Hyaluronoglucosaminidase
Horses
Spermatozoa
oocytes
spermatozoa
horses
Sperm Head
stallions
Calcium
calcium
Zona Pellucida
Epididymis
Exocytosis
Herpes Zoster
Hyaluronic Acid
Semen
hyaluronic acid
Fluorescent Antibody Technique

Keywords

  • Epididymis
  • Hyaluronidase
  • PH-20
  • Sperm
  • Stallion

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biotechnology
  • Genetics

Cite this

Equine sperm-oocyte interaction : The role of sperm surface hyaluronidase. / Meyers, Stuart A.

In: Animal Reproduction Science, Vol. 68, No. 3-4, 03.12.2001, p. 291-303.

Research output: Contribution to journalArticle

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