Equine Genital Squamous Cell Carcinoma: In Situ Hybridization Identifies a Distinct Subset Containing Equus caballus Papillomavirus 2

K. W. Zhu, Verena K Affolter, A. M. Gaynor, F. N. Dela Cruz, Patricia Pesavento

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Equus caballus papillomavirus 2 (EcPV2) has been proposed as an etiologic agent for genital squamous cell carcinoma (SCC), the most common malignant tumor of the horse penis. EcPV2 is commonly detected by polymerase chain reaction (PCR) on normal horse genitalia; therefore, unraveling the virus’ role in oncogenic transformation requires other methods of detection. In this study, a highly sensitive multiple-probe chromogenic in situ hybridization (ISH) technique was designed to recognize the E6/E7 oncogenes of EcPV2. ISH demonstrated abundant virus within 6 of 13 penile and preputial SCCs, whereas evidence of solar damage was found in 6 cases that were negative for EcPV2 by ISH. The ISH technique is valuable for studies of pathogenesis, since it demonstrates for the first time that the vast majority of neoplastic cells contain virus. Moreover, hybridization was present in all metastases examined, implying stability of E6/E7 expression in these clonal populations of neoplastic cells. This study contributes to the accumulating evidence for a causal role of EcPV2 in a subset of genital SCCs in horses.

Original languageEnglish (US)
Pages (from-to)1067-1072
Number of pages6
JournalVeterinary Pathology
Volume52
Issue number6
DOIs
StatePublished - Nov 1 2015

Fingerprint

Equine papillomavirus 1
Carcinoma in Situ
squamous cell carcinoma
in situ hybridization
Horses
genitalia
In Situ Hybridization
Squamous Cell Carcinoma
horses
viruses
Viruses
penis
oncogenes
metastasis
hybridization
pathogenesis
polymerase chain reaction
methodology
Genitalia
Penis

Keywords

  • equine
  • Equus caballus papillomavirus
  • genital
  • in situ hybridization
  • penis
  • solar
  • squamous cell carcinoma

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Equine Genital Squamous Cell Carcinoma : In Situ Hybridization Identifies a Distinct Subset Containing Equus caballus Papillomavirus 2. / Zhu, K. W.; Affolter, Verena K; Gaynor, A. M.; Dela Cruz, F. N.; Pesavento, Patricia.

In: Veterinary Pathology, Vol. 52, No. 6, 01.11.2015, p. 1067-1072.

Research output: Contribution to journalArticle

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