Equine bullous pemphigoid IgG autoantibodies target linear epitopes in the NC16A ectodomain of collagen XVII (BP180, BPAG2)

Thierry Olivry, Anna K G Borrillo, Luting Xu, Stanley M. Dunston, Nathan M. Slovis, Verena K Affolter, Terese C. Demanuelle, Lawrence S. Chan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Bullous pemphigoid (BP) is an autoimmune subepithelial blistering dermatosis of humans, dogs, cats and pigs. It is characterized by skin-fixed and circulating IgG autoantibodies that target one or both BP antigens. An immunological homologue of BP in humans was diagnosed in two horses with cutaneous and mucosal ulcerations as well as microscopic subepithelial vesiculation. Immunological investigations revealed similar findings for both the horses. Direct immunofluorescence demonstrated the presence of IgG deposited linearly at the dermoepidermal junction in mucosal and skin biopsy specimens. Indirect immunofluorescence testing confirmed the existence of circulating basement membrane-specific IgG autoantibodies. Using intact and salt-split epithelial substrates, serum IgG were shown to target antigens situated not only at the basal, but also at the lateral and apical aspects of stratum basale keratinocytes. Immunoblotting and ELISA corroborated that the IgG from affected horses, but not those from normal controls, exhibited high immunoreactivity against the NC16A extracellular domain of type XVII collagen (BPAG2, BP180). Equine BP could be proposed, therefore, as another spontaneous model of this most common basement membrane autoimmune dermatosis of humans. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)45-52
Number of pages8
JournalVeterinary Immunology and Immunopathology
Volume73
Issue number1
DOIs
StatePublished - Jan 31 2000

Fingerprint

Bullous Pemphigoid
autoantibodies
Autoantibodies
epitopes
Horses
collagen
Epitopes
Immunoglobulin G
horses
basement membrane
skin diseases
skin (animal)
fluorescent antibody technique
Skin Diseases
Basement Membrane
Skin
antigens
keratinocytes
Antigens
immunoblotting

Keywords

  • Autoimmunity
  • Basement membrane zone
  • Bullous pemphigoid
  • Horse

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

Cite this

Equine bullous pemphigoid IgG autoantibodies target linear epitopes in the NC16A ectodomain of collagen XVII (BP180, BPAG2). / Olivry, Thierry; Borrillo, Anna K G; Xu, Luting; Dunston, Stanley M.; Slovis, Nathan M.; Affolter, Verena K; Demanuelle, Terese C.; Chan, Lawrence S.

In: Veterinary Immunology and Immunopathology, Vol. 73, No. 1, 31.01.2000, p. 45-52.

Research output: Contribution to journalArticle

Olivry, Thierry ; Borrillo, Anna K G ; Xu, Luting ; Dunston, Stanley M. ; Slovis, Nathan M. ; Affolter, Verena K ; Demanuelle, Terese C. ; Chan, Lawrence S. / Equine bullous pemphigoid IgG autoantibodies target linear epitopes in the NC16A ectodomain of collagen XVII (BP180, BPAG2). In: Veterinary Immunology and Immunopathology. 2000 ; Vol. 73, No. 1. pp. 45-52.
@article{04762152f26b400d9060d05c1518bcc4,
title = "Equine bullous pemphigoid IgG autoantibodies target linear epitopes in the NC16A ectodomain of collagen XVII (BP180, BPAG2)",
abstract = "Bullous pemphigoid (BP) is an autoimmune subepithelial blistering dermatosis of humans, dogs, cats and pigs. It is characterized by skin-fixed and circulating IgG autoantibodies that target one or both BP antigens. An immunological homologue of BP in humans was diagnosed in two horses with cutaneous and mucosal ulcerations as well as microscopic subepithelial vesiculation. Immunological investigations revealed similar findings for both the horses. Direct immunofluorescence demonstrated the presence of IgG deposited linearly at the dermoepidermal junction in mucosal and skin biopsy specimens. Indirect immunofluorescence testing confirmed the existence of circulating basement membrane-specific IgG autoantibodies. Using intact and salt-split epithelial substrates, serum IgG were shown to target antigens situated not only at the basal, but also at the lateral and apical aspects of stratum basale keratinocytes. Immunoblotting and ELISA corroborated that the IgG from affected horses, but not those from normal controls, exhibited high immunoreactivity against the NC16A extracellular domain of type XVII collagen (BPAG2, BP180). Equine BP could be proposed, therefore, as another spontaneous model of this most common basement membrane autoimmune dermatosis of humans. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "Autoimmunity, Basement membrane zone, Bullous pemphigoid, Horse",
author = "Thierry Olivry and Borrillo, {Anna K G} and Luting Xu and Dunston, {Stanley M.} and Slovis, {Nathan M.} and Affolter, {Verena K} and Demanuelle, {Terese C.} and Chan, {Lawrence S.}",
year = "2000",
month = "1",
day = "31",
doi = "10.1016/S0165-2427(99)00151-8",
language = "English (US)",
volume = "73",
pages = "45--52",
journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Equine bullous pemphigoid IgG autoantibodies target linear epitopes in the NC16A ectodomain of collagen XVII (BP180, BPAG2)

AU - Olivry, Thierry

AU - Borrillo, Anna K G

AU - Xu, Luting

AU - Dunston, Stanley M.

AU - Slovis, Nathan M.

AU - Affolter, Verena K

AU - Demanuelle, Terese C.

AU - Chan, Lawrence S.

PY - 2000/1/31

Y1 - 2000/1/31

N2 - Bullous pemphigoid (BP) is an autoimmune subepithelial blistering dermatosis of humans, dogs, cats and pigs. It is characterized by skin-fixed and circulating IgG autoantibodies that target one or both BP antigens. An immunological homologue of BP in humans was diagnosed in two horses with cutaneous and mucosal ulcerations as well as microscopic subepithelial vesiculation. Immunological investigations revealed similar findings for both the horses. Direct immunofluorescence demonstrated the presence of IgG deposited linearly at the dermoepidermal junction in mucosal and skin biopsy specimens. Indirect immunofluorescence testing confirmed the existence of circulating basement membrane-specific IgG autoantibodies. Using intact and salt-split epithelial substrates, serum IgG were shown to target antigens situated not only at the basal, but also at the lateral and apical aspects of stratum basale keratinocytes. Immunoblotting and ELISA corroborated that the IgG from affected horses, but not those from normal controls, exhibited high immunoreactivity against the NC16A extracellular domain of type XVII collagen (BPAG2, BP180). Equine BP could be proposed, therefore, as another spontaneous model of this most common basement membrane autoimmune dermatosis of humans. Copyright (C) 2000 Elsevier Science B.V.

AB - Bullous pemphigoid (BP) is an autoimmune subepithelial blistering dermatosis of humans, dogs, cats and pigs. It is characterized by skin-fixed and circulating IgG autoantibodies that target one or both BP antigens. An immunological homologue of BP in humans was diagnosed in two horses with cutaneous and mucosal ulcerations as well as microscopic subepithelial vesiculation. Immunological investigations revealed similar findings for both the horses. Direct immunofluorescence demonstrated the presence of IgG deposited linearly at the dermoepidermal junction in mucosal and skin biopsy specimens. Indirect immunofluorescence testing confirmed the existence of circulating basement membrane-specific IgG autoantibodies. Using intact and salt-split epithelial substrates, serum IgG were shown to target antigens situated not only at the basal, but also at the lateral and apical aspects of stratum basale keratinocytes. Immunoblotting and ELISA corroborated that the IgG from affected horses, but not those from normal controls, exhibited high immunoreactivity against the NC16A extracellular domain of type XVII collagen (BPAG2, BP180). Equine BP could be proposed, therefore, as another spontaneous model of this most common basement membrane autoimmune dermatosis of humans. Copyright (C) 2000 Elsevier Science B.V.

KW - Autoimmunity

KW - Basement membrane zone

KW - Bullous pemphigoid

KW - Horse

UR - http://www.scopus.com/inward/record.url?scp=0033982506&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033982506&partnerID=8YFLogxK

U2 - 10.1016/S0165-2427(99)00151-8

DO - 10.1016/S0165-2427(99)00151-8

M3 - Article

C2 - 10678397

AN - SCOPUS:0033982506

VL - 73

SP - 45

EP - 52

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 1

ER -