Equine bullous pemphigoid IgG autoantibodies target linear epitopes in the NC16A ectodomain of collagen XVII (BP180, BPAG2)

Thierry Olivry, Anna K G Borrillo, Luting Xu, Stanley M. Dunston, Nathan M. Slovis, Verena K Affolter, Terese C. Demanuelle, Lawrence S. Chan

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Bullous pemphigoid (BP) is an autoimmune subepithelial blistering dermatosis of humans, dogs, cats and pigs. It is characterized by skin-fixed and circulating IgG autoantibodies that target one or both BP antigens. An immunological homologue of BP in humans was diagnosed in two horses with cutaneous and mucosal ulcerations as well as microscopic subepithelial vesiculation. Immunological investigations revealed similar findings for both the horses. Direct immunofluorescence demonstrated the presence of IgG deposited linearly at the dermoepidermal junction in mucosal and skin biopsy specimens. Indirect immunofluorescence testing confirmed the existence of circulating basement membrane-specific IgG autoantibodies. Using intact and salt-split epithelial substrates, serum IgG were shown to target antigens situated not only at the basal, but also at the lateral and apical aspects of stratum basale keratinocytes. Immunoblotting and ELISA corroborated that the IgG from affected horses, but not those from normal controls, exhibited high immunoreactivity against the NC16A extracellular domain of type XVII collagen (BPAG2, BP180). Equine BP could be proposed, therefore, as another spontaneous model of this most common basement membrane autoimmune dermatosis of humans. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)45-52
Number of pages8
JournalVeterinary Immunology and Immunopathology
Volume73
Issue number1
DOIs
StatePublished - Jan 31 2000

Keywords

  • Autoimmunity
  • Basement membrane zone
  • Bullous pemphigoid
  • Horse

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

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