Equine bone marrow volume reduction, red blood cell depletion, and mononuclear cell recovery using the PrepaCyte-CB processing system

Andrew G. Burton, Kaitlin C. Clark, Dori L Borjesson, Danielle D. Carrade, Julie Burges, Sean D Owens

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Volume reduction and RBC depletion of equine bone marrow specimens are necessary processing steps for the immediate therapeutic use of bone marrow (BM)-derived mesenchymal stem cells (MSC), and for MSC expansion in culture. Objectives: The purpose of the study was to evaluate the ability of the PrepaCyte-CB processing system to reduce volume, deplete RBC, and recover mononuclear cells (MNC) from equine BM specimens. Methods: One hundred and twenty mL of heparinized BM were obtained from each of 90 horses. A CBC was performed on the BM pre- and post-PrepaCyte-CB processing. Volume and RBC reduction, and total nucleated cell (TNC) and MNC recoveries were determined. Results: Bone marrow volume was reduced from 120 mL to 21 mL with a median RBC depletion of 90.1% (range, 62.0-96.7%). The median preprocessing total TNC count was 2.2 × 10<sup>9</sup> (range, 0.46-7.9 × 10<sup>9</sup>) and the median postprocessing TNC count was 1.7 × 10<sup>9</sup> (range, 0.3-4.4 × 10<sup>9</sup>; P < .0001), with a median recovery of 73.5% (range, 22.4-216.7%). The median preprocessing total MNC count was 0.9 × 10<sup>9</sup> (range, 0.1-4.7 × 10<sup>9</sup>) and median postprocessing total MNC count was 0.8 × 10<sup>9</sup> (range, 0.1-2.7 × 10<sup>9</sup>; P = .06), with a median recovery of 83.7% (range, 15.4-413.9%). Conclusions: The PrepaCyte-CB processing system can be used to deplete both volume and RBC, and recover MNC from equine BM specimens. Further studies assessing the viability of MSC and the efficacy of MSC expansion after using the PrepaCyte-CB processing system are warranted.

Original languageEnglish (US)
Pages (from-to)188-193
Number of pages6
JournalVeterinary Clinical Pathology
Volume44
Issue number2
DOIs
StatePublished - Jun 1 2015

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bone marrow
Horses
Mesenchymal Stromal Cells
erythrocytes
Erythrocytes
Bone Marrow
horses
stem cells
Cell Count
Bone Marrow Cells
cells
Therapeutic Uses
viability
therapeutics

Keywords

  • Cell separation
  • Horses
  • Mesenchymal stem cell
  • Regenerative

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Equine bone marrow volume reduction, red blood cell depletion, and mononuclear cell recovery using the PrepaCyte-CB processing system. / Burton, Andrew G.; Clark, Kaitlin C.; Borjesson, Dori L; Carrade, Danielle D.; Burges, Julie; Owens, Sean D.

In: Veterinary Clinical Pathology, Vol. 44, No. 2, 01.06.2015, p. 188-193.

Research output: Contribution to journalArticle

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abstract = "Background: Volume reduction and RBC depletion of equine bone marrow specimens are necessary processing steps for the immediate therapeutic use of bone marrow (BM)-derived mesenchymal stem cells (MSC), and for MSC expansion in culture. Objectives: The purpose of the study was to evaluate the ability of the PrepaCyte-CB processing system to reduce volume, deplete RBC, and recover mononuclear cells (MNC) from equine BM specimens. Methods: One hundred and twenty mL of heparinized BM were obtained from each of 90 horses. A CBC was performed on the BM pre- and post-PrepaCyte-CB processing. Volume and RBC reduction, and total nucleated cell (TNC) and MNC recoveries were determined. Results: Bone marrow volume was reduced from 120 mL to 21 mL with a median RBC depletion of 90.1{\%} (range, 62.0-96.7{\%}). The median preprocessing total TNC count was 2.2 × 109 (range, 0.46-7.9 × 109) and the median postprocessing TNC count was 1.7 × 109 (range, 0.3-4.4 × 109; P < .0001), with a median recovery of 73.5{\%} (range, 22.4-216.7{\%}). The median preprocessing total MNC count was 0.9 × 109 (range, 0.1-4.7 × 109) and median postprocessing total MNC count was 0.8 × 109 (range, 0.1-2.7 × 109; P = .06), with a median recovery of 83.7{\%} (range, 15.4-413.9{\%}). Conclusions: The PrepaCyte-CB processing system can be used to deplete both volume and RBC, and recover MNC from equine BM specimens. Further studies assessing the viability of MSC and the efficacy of MSC expansion after using the PrepaCyte-CB processing system are warranted.",
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T1 - Equine bone marrow volume reduction, red blood cell depletion, and mononuclear cell recovery using the PrepaCyte-CB processing system

AU - Burton, Andrew G.

AU - Clark, Kaitlin C.

AU - Borjesson, Dori L

AU - Carrade, Danielle D.

AU - Burges, Julie

AU - Owens, Sean D

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N2 - Background: Volume reduction and RBC depletion of equine bone marrow specimens are necessary processing steps for the immediate therapeutic use of bone marrow (BM)-derived mesenchymal stem cells (MSC), and for MSC expansion in culture. Objectives: The purpose of the study was to evaluate the ability of the PrepaCyte-CB processing system to reduce volume, deplete RBC, and recover mononuclear cells (MNC) from equine BM specimens. Methods: One hundred and twenty mL of heparinized BM were obtained from each of 90 horses. A CBC was performed on the BM pre- and post-PrepaCyte-CB processing. Volume and RBC reduction, and total nucleated cell (TNC) and MNC recoveries were determined. Results: Bone marrow volume was reduced from 120 mL to 21 mL with a median RBC depletion of 90.1% (range, 62.0-96.7%). The median preprocessing total TNC count was 2.2 × 109 (range, 0.46-7.9 × 109) and the median postprocessing TNC count was 1.7 × 109 (range, 0.3-4.4 × 109; P < .0001), with a median recovery of 73.5% (range, 22.4-216.7%). The median preprocessing total MNC count was 0.9 × 109 (range, 0.1-4.7 × 109) and median postprocessing total MNC count was 0.8 × 109 (range, 0.1-2.7 × 109; P = .06), with a median recovery of 83.7% (range, 15.4-413.9%). Conclusions: The PrepaCyte-CB processing system can be used to deplete both volume and RBC, and recover MNC from equine BM specimens. Further studies assessing the viability of MSC and the efficacy of MSC expansion after using the PrepaCyte-CB processing system are warranted.

AB - Background: Volume reduction and RBC depletion of equine bone marrow specimens are necessary processing steps for the immediate therapeutic use of bone marrow (BM)-derived mesenchymal stem cells (MSC), and for MSC expansion in culture. Objectives: The purpose of the study was to evaluate the ability of the PrepaCyte-CB processing system to reduce volume, deplete RBC, and recover mononuclear cells (MNC) from equine BM specimens. Methods: One hundred and twenty mL of heparinized BM were obtained from each of 90 horses. A CBC was performed on the BM pre- and post-PrepaCyte-CB processing. Volume and RBC reduction, and total nucleated cell (TNC) and MNC recoveries were determined. Results: Bone marrow volume was reduced from 120 mL to 21 mL with a median RBC depletion of 90.1% (range, 62.0-96.7%). The median preprocessing total TNC count was 2.2 × 109 (range, 0.46-7.9 × 109) and the median postprocessing TNC count was 1.7 × 109 (range, 0.3-4.4 × 109; P < .0001), with a median recovery of 73.5% (range, 22.4-216.7%). The median preprocessing total MNC count was 0.9 × 109 (range, 0.1-4.7 × 109) and median postprocessing total MNC count was 0.8 × 109 (range, 0.1-2.7 × 109; P = .06), with a median recovery of 83.7% (range, 15.4-413.9%). Conclusions: The PrepaCyte-CB processing system can be used to deplete both volume and RBC, and recover MNC from equine BM specimens. Further studies assessing the viability of MSC and the efficacy of MSC expansion after using the PrepaCyte-CB processing system are warranted.

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