Abstract
The equine bone marrow responds to blood loss by increased erythropoiesis, only releasing reticulocytes into the peripheral circulation in severe chronic anemia. We have used morphometric analysis based on electron microscopy of the equine marrow to examine the maturation and release of reticulocytes. Developing red cells in the bone marrow of normal and chronically anemic horses were divided into four stages: early, intermediate, late-stage erythroblasts, and reticulocytes. Morphometric analysis of each stage included volume density of mitochondria per μm3 of cytoplasm, surface area of the outer mitochondrial membrane per unit volume of mitochondria, and the number of ribosomes per unit volume of cytoplasm (total, clustered, single). Matched t tests between normal and anemic animals showed significant differences (P ≤ .001) for volume density of mitochondria and numbers of ribosomes only at the reticulocyte stage. The large reticulocyte produced and released in chronic anemia may be best explained by a skipped mitotic division.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 202-206 |
| Number of pages | 5 |
| Journal | Anatomical Record |
| Volume | 213 |
| Issue number | 2 |
| State | Published - 1985 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Anatomy
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Equine bone marrow : A quantitative analysis of erythroid maturation. / Tablin, Fern; Weiss, L.
In: Anatomical Record, Vol. 213, No. 2, 1985, p. 202-206.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Equine bone marrow
T2 - A quantitative analysis of erythroid maturation
AU - Tablin, Fern
AU - Weiss, L.
PY - 1985
Y1 - 1985
N2 - The equine bone marrow responds to blood loss by increased erythropoiesis, only releasing reticulocytes into the peripheral circulation in severe chronic anemia. We have used morphometric analysis based on electron microscopy of the equine marrow to examine the maturation and release of reticulocytes. Developing red cells in the bone marrow of normal and chronically anemic horses were divided into four stages: early, intermediate, late-stage erythroblasts, and reticulocytes. Morphometric analysis of each stage included volume density of mitochondria per μm3 of cytoplasm, surface area of the outer mitochondrial membrane per unit volume of mitochondria, and the number of ribosomes per unit volume of cytoplasm (total, clustered, single). Matched t tests between normal and anemic animals showed significant differences (P ≤ .001) for volume density of mitochondria and numbers of ribosomes only at the reticulocyte stage. The large reticulocyte produced and released in chronic anemia may be best explained by a skipped mitotic division.
AB - The equine bone marrow responds to blood loss by increased erythropoiesis, only releasing reticulocytes into the peripheral circulation in severe chronic anemia. We have used morphometric analysis based on electron microscopy of the equine marrow to examine the maturation and release of reticulocytes. Developing red cells in the bone marrow of normal and chronically anemic horses were divided into four stages: early, intermediate, late-stage erythroblasts, and reticulocytes. Morphometric analysis of each stage included volume density of mitochondria per μm3 of cytoplasm, surface area of the outer mitochondrial membrane per unit volume of mitochondria, and the number of ribosomes per unit volume of cytoplasm (total, clustered, single). Matched t tests between normal and anemic animals showed significant differences (P ≤ .001) for volume density of mitochondria and numbers of ribosomes only at the reticulocyte stage. The large reticulocyte produced and released in chronic anemia may be best explained by a skipped mitotic division.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022384005&partnerID=8YFLogxK
M3 - Article
C2 - 4073571
AN - SCOPUS:0022384005
VL - 213
SP - 202
EP - 206
JO - Anatomical Record
JF - Anatomical Record
SN - 1932-8486
IS - 2
ER -