Abstract
NFκB activity analysis was performed with pNFκB-luc reporter plasmid. Expression of NFκB in nuclear and expression of Igκ in intact cells were analyzed by Western blot to identify whether Epstein-Barr virus encoded latent membrane protein 1 (LMP1) increases the expression of immunoglobulin κ light chain through NFκB signal transduction pathway in nasopharyngeal carcinoma. Results showed that LMP1 could increase NFκB activity and promote the accumulation of NFκB p65 in nucleus, and LMP1 also increased Igκ expression. In addition, phosphorothioate oligonucleotides to antisense NFκB p65 and p50 inhibited the Igκ expression. These imply that LMP1 can regulate Igκ expression via NFκB signal transduction pathway in nasopharyngeal carcinoma.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 662-663 |
| Number of pages | 2 |
| Journal | Acta Biochimica et Biophysica Sinica |
| Volume | 31 |
| Issue number | 6 |
| State | Published - 1999 |
| Externally published | Yes |
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Keywords
- EBV encoded LMP1
- Igκ
- Nasopharyngeal carcinoma
- NFκ:B
ASJC Scopus subject areas
- Biochemistry
- Biophysics
Cite this
Epstein-Barr virus encoded latent membrane protein 1 increases expression of immimoglobulin κ light chain through NFκB in a nasopharyngeal carcinoma cell line. / Liao, Wei; Tang, Ming; Li, Jian-Jian; Leo, M. Lee; Cao, Ya.
In: Acta Biochimica et Biophysica Sinica, Vol. 31, No. 6, 1999, p. 662-663.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Epstein-Barr virus encoded latent membrane protein 1 increases expression of immimoglobulin κ light chain through NFκB in a nasopharyngeal carcinoma cell line
AU - Liao, Wei
AU - Tang, Ming
AU - Li, Jian-Jian
AU - Leo, M. Lee
AU - Cao, Ya
PY - 1999
Y1 - 1999
N2 - NFκB activity analysis was performed with pNFκB-luc reporter plasmid. Expression of NFκB in nuclear and expression of Igκ in intact cells were analyzed by Western blot to identify whether Epstein-Barr virus encoded latent membrane protein 1 (LMP1) increases the expression of immunoglobulin κ light chain through NFκB signal transduction pathway in nasopharyngeal carcinoma. Results showed that LMP1 could increase NFκB activity and promote the accumulation of NFκB p65 in nucleus, and LMP1 also increased Igκ expression. In addition, phosphorothioate oligonucleotides to antisense NFκB p65 and p50 inhibited the Igκ expression. These imply that LMP1 can regulate Igκ expression via NFκB signal transduction pathway in nasopharyngeal carcinoma.
AB - NFκB activity analysis was performed with pNFκB-luc reporter plasmid. Expression of NFκB in nuclear and expression of Igκ in intact cells were analyzed by Western blot to identify whether Epstein-Barr virus encoded latent membrane protein 1 (LMP1) increases the expression of immunoglobulin κ light chain through NFκB signal transduction pathway in nasopharyngeal carcinoma. Results showed that LMP1 could increase NFκB activity and promote the accumulation of NFκB p65 in nucleus, and LMP1 also increased Igκ expression. In addition, phosphorothioate oligonucleotides to antisense NFκB p65 and p50 inhibited the Igκ expression. These imply that LMP1 can regulate Igκ expression via NFκB signal transduction pathway in nasopharyngeal carcinoma.
KW - EBV encoded LMP1
KW - Igκ
KW - Nasopharyngeal carcinoma
KW - NFκ:B
UR - http://www.scopus.com/inward/record.url?scp=20444395124&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20444395124&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:20444395124
VL - 31
SP - 662
EP - 663
JO - Acta Biochimica et Biophysica Sinica
JF - Acta Biochimica et Biophysica Sinica
SN - 1672-9145
IS - 6
ER -