Epstein-Barr virus encoded latent membrane protein 1 increases expression of immimoglobulin κ light chain through NFκB in a nasopharyngeal carcinoma cell line

NFκB activity analysis was performed with pNFκB-luc reporter plasmid. Expression of NFκB in nuclear and expression of Igκ in intact cells were analyzed by Western blot to identify whether Epstein-Barr virus encoded latent membrane protein 1 (LMP1) increases the expression of immunoglobulin κ light chain through NFκB signal transduction pathway in nasopharyngeal carcinoma. Results showed that LMP1 could increase NFκB activity and promote the accumulation of NFκB p65 in nucleus, and LMP1 also increased Igκ expression. In addition, phosphorothioate oligonucleotides to antisense NFκB p65 and p50 inhibited the Igκ expression. These imply that LMP1 can regulate Igκ expression via NFκB signal transduction pathway in nasopharyngeal carcinoma.