Epstein-Barr virus encoded latent membrane protein 1 increases expression of immimoglobulin κ light chain through NFκB in a nasopharyngeal carcinoma cell line

Wei Liao, Ming Tang, Jian-Jian Li, M. Lee Leo, Ya Cao

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

NFκB activity analysis was performed with pNFκB-luc reporter plasmid. Expression of NFκB in nuclear and expression of Igκ in intact cells were analyzed by Western blot to identify whether Epstein-Barr virus encoded latent membrane protein 1 (LMP1) increases the expression of immunoglobulin κ light chain through NFκB signal transduction pathway in nasopharyngeal carcinoma. Results showed that LMP1 could increase NFκB activity and promote the accumulation of NFκB p65 in nucleus, and LMP1 also increased Igκ expression. In addition, phosphorothioate oligonucleotides to antisense NFκB p65 and p50 inhibited the Igκ expression. These imply that LMP1 can regulate Igκ expression via NFκB signal transduction pathway in nasopharyngeal carcinoma.

Original languageEnglish (US)
Pages (from-to)662-663
Number of pages2
JournalActa Biochimica et Biophysica Sinica
Volume31
Issue number6
StatePublished - 1999
Externally publishedYes

Fingerprint

Viruses
Membrane Proteins
Cells
Light
Cell Line
Signal transduction
Signal Transduction
Phosphorothioate Oligonucleotides
Immunoglobulin Light Chains
Plasmids
Western Blotting
Nasopharyngeal carcinoma
Epstein-Barr virus EBV-associated membrane antigen

Keywords

  • EBV encoded LMP1
  • Igκ
  • Nasopharyngeal carcinoma
  • NFκ:B

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

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title = "Epstein-Barr virus encoded latent membrane protein 1 increases expression of immimoglobulin κ light chain through NFκB in a nasopharyngeal carcinoma cell line",
abstract = "NFκB activity analysis was performed with pNFκB-luc reporter plasmid. Expression of NFκB in nuclear and expression of Igκ in intact cells were analyzed by Western blot to identify whether Epstein-Barr virus encoded latent membrane protein 1 (LMP1) increases the expression of immunoglobulin κ light chain through NFκB signal transduction pathway in nasopharyngeal carcinoma. Results showed that LMP1 could increase NFκB activity and promote the accumulation of NFκB p65 in nucleus, and LMP1 also increased Igκ expression. In addition, phosphorothioate oligonucleotides to antisense NFκB p65 and p50 inhibited the Igκ expression. These imply that LMP1 can regulate Igκ expression via NFκB signal transduction pathway in nasopharyngeal carcinoma.",
keywords = "EBV encoded LMP1, Igκ, Nasopharyngeal carcinoma, NFκ:B",
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journal = "Acta Biochimica et Biophysica Sinica",
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T1 - Epstein-Barr virus encoded latent membrane protein 1 increases expression of immimoglobulin κ light chain through NFκB in a nasopharyngeal carcinoma cell line

AU - Liao, Wei

AU - Tang, Ming

AU - Li, Jian-Jian

AU - Leo, M. Lee

AU - Cao, Ya

PY - 1999

Y1 - 1999

N2 - NFκB activity analysis was performed with pNFκB-luc reporter plasmid. Expression of NFκB in nuclear and expression of Igκ in intact cells were analyzed by Western blot to identify whether Epstein-Barr virus encoded latent membrane protein 1 (LMP1) increases the expression of immunoglobulin κ light chain through NFκB signal transduction pathway in nasopharyngeal carcinoma. Results showed that LMP1 could increase NFκB activity and promote the accumulation of NFκB p65 in nucleus, and LMP1 also increased Igκ expression. In addition, phosphorothioate oligonucleotides to antisense NFκB p65 and p50 inhibited the Igκ expression. These imply that LMP1 can regulate Igκ expression via NFκB signal transduction pathway in nasopharyngeal carcinoma.

AB - NFκB activity analysis was performed with pNFκB-luc reporter plasmid. Expression of NFκB in nuclear and expression of Igκ in intact cells were analyzed by Western blot to identify whether Epstein-Barr virus encoded latent membrane protein 1 (LMP1) increases the expression of immunoglobulin κ light chain through NFκB signal transduction pathway in nasopharyngeal carcinoma. Results showed that LMP1 could increase NFκB activity and promote the accumulation of NFκB p65 in nucleus, and LMP1 also increased Igκ expression. In addition, phosphorothioate oligonucleotides to antisense NFκB p65 and p50 inhibited the Igκ expression. These imply that LMP1 can regulate Igκ expression via NFκB signal transduction pathway in nasopharyngeal carcinoma.

KW - EBV encoded LMP1

KW - Igκ

KW - Nasopharyngeal carcinoma

KW - NFκ:B

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