Epoxide hydrolase activities in Drosophila melanogaster

L. G. Harshman, J. Casas, E. C. Dietze, B. D. Hammock

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Epoxide hydrolase (EH) activity in Drosophila melanogaster cell fractions was characterized using juvenile hormone (JH III) and cis-stilbene oxide (CSO) as substrates. A comparison of detergents indicated that 0.3% Lubrol PX was relatively effective for solubilizing EH activity from the 20,000 and 100,000 g pellets. The effect of inhibitors, pH, temperature, salt and organic solvents on EH activity depended on the substrate and cell fraction tested, which suggested there were multiple activities present. For initial purification, polyethylene glycol was useful for precipitating EH activity from the 100,000 g supernatant.

Original languageEnglish (US)
Pages (from-to)887-894
Number of pages8
JournalInsect Biochemistry
Volume21
Issue number8
DOIs
StatePublished - 1991

Fingerprint

epoxide hydrolase
Epoxide Hydrolases
Drosophila melanogaster
Juvenile Hormones
stilbenes
juvenile hormones
Substrates
polyethylene glycol
solubilization
detergents
Detergents
Organic solvents
oxides
Purification
pellets
Salts
cells
salts
Temperature
temperature

Keywords

  • Drosophila melanogaster
  • epoxide hydrolase
  • juvenile hormone

Cite this

Harshman, L. G., Casas, J., Dietze, E. C., & Hammock, B. D. (1991). Epoxide hydrolase activities in Drosophila melanogaster. Insect Biochemistry, 21(8), 887-894. https://doi.org/10.1016/0020-1790(91)90095-V

Epoxide hydrolase activities in Drosophila melanogaster. / Harshman, L. G.; Casas, J.; Dietze, E. C.; Hammock, B. D.

In: Insect Biochemistry, Vol. 21, No. 8, 1991, p. 887-894.

Research output: Contribution to journalArticle

Harshman, LG, Casas, J, Dietze, EC & Hammock, BD 1991, 'Epoxide hydrolase activities in Drosophila melanogaster', Insect Biochemistry, vol. 21, no. 8, pp. 887-894. https://doi.org/10.1016/0020-1790(91)90095-V
Harshman, L. G. ; Casas, J. ; Dietze, E. C. ; Hammock, B. D. / Epoxide hydrolase activities in Drosophila melanogaster. In: Insect Biochemistry. 1991 ; Vol. 21, No. 8. pp. 887-894.
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