Epitope mapping of pneumococcal surface protein A of strain Rx1 using monoclonal antibodies and molecular structure modelling

Jan Kolberg, Audun Aase, Gunnhild Rødal, James E Littlejohn, Mark J. Jedrzejas

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Pneumococcal surface protein A (PspA) is an antigenic variable vaccine candidate of Streptococcus pneumoniae. Epitope similarities between PspA from the American vaccine candidate strain Rx1 and Norwegian clinical isolates were studied using PspA specific monoclonal antibodies (mAbs) made against clinical Norwegian strains. Using recombinant PspA/Rx1 fragments and immunoblotting the epitopes for mAbs were mapped to two regions of amino acids, 1-67 and 67-236. The discovered epitopes were visualized by modelling of the PspA:Fab part of mAb in three dimensions. Flow cytometric analysis showed that the epitopes for majority of mAbs were accessible for antibody binding on live pneumococci. Also, the epitopes for majority of the mAbs are widely expressed among clinical Norwegian isolates.

Original languageEnglish (US)
Pages (from-to)265-273
Number of pages9
JournalFEMS Immunology and Medical Microbiology
Volume39
Issue number3
DOIs
StatePublished - Dec 5 2003
Externally publishedYes

Keywords

  • Monoclonal antibody
  • Pneumococcal surface protein A
  • Pneumococcal vaccine
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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