Abstract
Pneumococcal surface protein A (PspA) is an antigenic variable vaccine candidate of Streptococcus pneumoniae. Epitope similarities between PspA from the American vaccine candidate strain Rx1 and Norwegian clinical isolates were studied using PspA specific monoclonal antibodies (mAbs) made against clinical Norwegian strains. Using recombinant PspA/Rx1 fragments and immunoblotting the epitopes for mAbs were mapped to two regions of amino acids, 1-67 and 67-236. The discovered epitopes were visualized by modelling of the PspA:Fab part of mAb in three dimensions. Flow cytometric analysis showed that the epitopes for majority of mAbs were accessible for antibody binding on live pneumococci. Also, the epitopes for majority of the mAbs are widely expressed among clinical Norwegian isolates.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 265-273 |
| Number of pages | 9 |
| Journal | FEMS Immunology and Medical Microbiology |
| Volume | 39 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 5 2003 |
| Externally published | Yes |
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Keywords
- Monoclonal antibody
- Pneumococcal surface protein A
- Pneumococcal vaccine
- Streptococcus pneumoniae
ASJC Scopus subject areas
- Immunology and Allergy
- Microbiology
- Immunology
- Microbiology (medical)
- Infectious Diseases
Cite this
Epitope mapping of pneumococcal surface protein A of strain Rx1 using monoclonal antibodies and molecular structure modelling. / Kolberg, Jan; Aase, Audun; Rødal, Gunnhild; Littlejohn, James E; Jedrzejas, Mark J.
In: FEMS Immunology and Medical Microbiology, Vol. 39, No. 3, 05.12.2003, p. 265-273.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Epitope mapping of pneumococcal surface protein A of strain Rx1 using monoclonal antibodies and molecular structure modelling
AU - Kolberg, Jan
AU - Aase, Audun
AU - Rødal, Gunnhild
AU - Littlejohn, James E
AU - Jedrzejas, Mark J.
PY - 2003/12/5
Y1 - 2003/12/5
N2 - Pneumococcal surface protein A (PspA) is an antigenic variable vaccine candidate of Streptococcus pneumoniae. Epitope similarities between PspA from the American vaccine candidate strain Rx1 and Norwegian clinical isolates were studied using PspA specific monoclonal antibodies (mAbs) made against clinical Norwegian strains. Using recombinant PspA/Rx1 fragments and immunoblotting the epitopes for mAbs were mapped to two regions of amino acids, 1-67 and 67-236. The discovered epitopes were visualized by modelling of the PspA:Fab part of mAb in three dimensions. Flow cytometric analysis showed that the epitopes for majority of mAbs were accessible for antibody binding on live pneumococci. Also, the epitopes for majority of the mAbs are widely expressed among clinical Norwegian isolates.
AB - Pneumococcal surface protein A (PspA) is an antigenic variable vaccine candidate of Streptococcus pneumoniae. Epitope similarities between PspA from the American vaccine candidate strain Rx1 and Norwegian clinical isolates were studied using PspA specific monoclonal antibodies (mAbs) made against clinical Norwegian strains. Using recombinant PspA/Rx1 fragments and immunoblotting the epitopes for mAbs were mapped to two regions of amino acids, 1-67 and 67-236. The discovered epitopes were visualized by modelling of the PspA:Fab part of mAb in three dimensions. Flow cytometric analysis showed that the epitopes for majority of mAbs were accessible for antibody binding on live pneumococci. Also, the epitopes for majority of the mAbs are widely expressed among clinical Norwegian isolates.
KW - Monoclonal antibody
KW - Pneumococcal surface protein A
KW - Pneumococcal vaccine
KW - Streptococcus pneumoniae
UR - http://www.scopus.com/inward/record.url?scp=0344513364&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0344513364&partnerID=8YFLogxK
U2 - 10.1016/S0928-8244(03)00255-4
DO - 10.1016/S0928-8244(03)00255-4
M3 - Article
C2 - 14642312
AN - SCOPUS:0344513364
VL - 39
SP - 265
EP - 273
JO - Pathogens and Disease
JF - Pathogens and Disease
SN - 2049-632X
IS - 3
ER -