Epitheliocystis hyperinfection in captive spotted eagle rays Aetobatus narinari associated with a novel Chlamydiales 16S rDNA signature sequence

Alvin Camus, Esteban Soto Martinez, Aimee Berliner, Tonya Clauss, Susan Sanchez

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This report details 2 cases of epitheliocystis in spotted eagle rays Aetobatus narinari associated with a novel Chlamydiales 16S rDNA signature sequence. Epitheliocystis is a common disease of variable severity affecting >50 species of wild and cultured freshwater and marine teleosts. Disease in elasmobranchs is rarely reported and descriptions are limited. Occurring in gill and skin epithelium, lesions are characterized by large hypertrophied cells with basophilic inclusions containing Gram-negative, chlamydia-like bacteria. Acute lethargy, labored respiration, and abnormal swimming developed in a captive spotted eagle ray following an uneventful quarantine period, and mild epitheliocystis lesions were found microscopically. Three months later, a second animal exhibited similar signs. A gill clip revealed myriad spherical bodies identical to the previous case, and treatment with chloramphenicol and oxytetracycline was initiated. Despite therapy, respiration became irregular and euthanasia was elected. Histologically, epitheliocystis inclusions up to 200 μm filled approximately 80% of lamellar troughs. Multifocal mild hypertrophy and hyperplasia of lamellar tips was accompanied by mild to moderate infiltrates of granulocytes and lymphocytes. Electron microscopy revealed a homogeneous population of elongate chlamydia-like bacterial forms similar in size and morphology to the primary long cells described in teleosts. Immunohistochemical staining with a polyclonal anti-chlamydial lipopolysaccharide antibody was positive. Sequence analysis of a unique 296 bp Chlamydiales signature sequence amplicon isolated from the rays showed greatest homology (85 to 87%) to 'Candidatus Piscichlamydia salmonis'.

Original languageEnglish (US)
Pages (from-to)13-21
Number of pages9
JournalDiseases of Aquatic Organisms
Volume104
Issue number1
DOIs
StatePublished - Apr 29 2013
Externally publishedYes

Fingerprint

Chlamydiales
teleost
lesion
respiration
Chlamydia
lesions (animal)
oxytetracycline
quarantine
breathing
gills
electron microscopy
homology
antibody
skin
trough
granulocytes
euthanasia
chloramphenicol
hypertrophy
hyperplasia

Keywords

  • 'Candidatus Piscichlamydia salmonis'
  • Diagnosis
  • Elasmobranch
  • Epithelium
  • Hypertrophy
  • Immunohistology
  • Lesion

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Epitheliocystis hyperinfection in captive spotted eagle rays Aetobatus narinari associated with a novel Chlamydiales 16S rDNA signature sequence. / Camus, Alvin; Soto Martinez, Esteban; Berliner, Aimee; Clauss, Tonya; Sanchez, Susan.

In: Diseases of Aquatic Organisms, Vol. 104, No. 1, 29.04.2013, p. 13-21.

Research output: Contribution to journalArticle

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abstract = "This report details 2 cases of epitheliocystis in spotted eagle rays Aetobatus narinari associated with a novel Chlamydiales 16S rDNA signature sequence. Epitheliocystis is a common disease of variable severity affecting >50 species of wild and cultured freshwater and marine teleosts. Disease in elasmobranchs is rarely reported and descriptions are limited. Occurring in gill and skin epithelium, lesions are characterized by large hypertrophied cells with basophilic inclusions containing Gram-negative, chlamydia-like bacteria. Acute lethargy, labored respiration, and abnormal swimming developed in a captive spotted eagle ray following an uneventful quarantine period, and mild epitheliocystis lesions were found microscopically. Three months later, a second animal exhibited similar signs. A gill clip revealed myriad spherical bodies identical to the previous case, and treatment with chloramphenicol and oxytetracycline was initiated. Despite therapy, respiration became irregular and euthanasia was elected. Histologically, epitheliocystis inclusions up to 200 μm filled approximately 80{\%} of lamellar troughs. Multifocal mild hypertrophy and hyperplasia of lamellar tips was accompanied by mild to moderate infiltrates of granulocytes and lymphocytes. Electron microscopy revealed a homogeneous population of elongate chlamydia-like bacterial forms similar in size and morphology to the primary long cells described in teleosts. Immunohistochemical staining with a polyclonal anti-chlamydial lipopolysaccharide antibody was positive. Sequence analysis of a unique 296 bp Chlamydiales signature sequence amplicon isolated from the rays showed greatest homology (85 to 87{\%}) to 'Candidatus Piscichlamydia salmonis'.",
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