Epigenetic regulation of human hedgehog interacting protein in glioma cell lines and primary tumor samples

Mehdi H. Shahi, Idoya Zazpe, Mohammad Afzal, Subrata Sinha, Robert B Rebhun, Bárbara Meléndez, Juan A. Rey, Javier S. Castresana

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Glioma constitutes one of the most common groups of brain tumors, and its prognosis is influenced by different genetic and epigenetic modulations. In this study, we demonstrated low or no expression of hedgehog interacting protein (HHIP) in most of the cell lines and primary glioma tumor samples. We further proceeded to promoter methylation study of this gene in the same cell lines and primary tumor samples and found 87 % (7/8) HHIP methylation in glioblastoma cell lines and 75 % (33/44) in primary tumor samples. These methylation pattern correlates with low or unexpressed HHIP in both cell lines and primary tumor samples. Our results suggest the possibility of epigenetic regulation of this gene in glioma, similarly to medulloblastoma, gastric, hepatic, and pancreatic cancers. Also, HHIP might be a diagnostic or prognostic marker in glioma and help to the detection of these tumors in early stages of disease.

Original languageEnglish (US)
Pages (from-to)2383-2391
Number of pages9
JournalTumor Biology
Volume36
Issue number4
DOIs
StatePublished - Apr 1 2015

ASJC Scopus subject areas

  • Medicine(all)

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    Shahi, M. H., Zazpe, I., Afzal, M., Sinha, S., Rebhun, R. B., Meléndez, B., Rey, J. A., & Castresana, J. S. (2015). Epigenetic regulation of human hedgehog interacting protein in glioma cell lines and primary tumor samples. Tumor Biology, 36(4), 2383-2391. https://doi.org/10.1007/s13277-014-2846-4