Epidermal growth factor modulates tyrosine phosphorylation of a novel tensin family member, tensin3

Yumin Cui, Yi Chun Liao, Su Hao Lo

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Here, we report the identification of a new tensin family member, tensin3, and its role in epidermal growth factor (EGF) signaling pathway. Human tensin3 cDNA encodes a 1445 amino acid sequence that shares extensive homology with tensin1, tensin2, and COOH-terminal tensin-like protein. Tensin3 is expressed in various tissues and in different cell types such as endothelia, epithelia, and fibroblasts. The potential role of tensin3 in EGF-induced signaling pathway is explored. EGF induces tyrosine phosphorylation of tensin3 in MDA-MB-468 cells in a time- and dose-dependent manner, but it is independent of an intact actin cytoskeleton or phosphatidylinositol 3-kinase. Activation of EGF receptor is necessary but not sufficient for tyrosine phosphorylation of tensin3. It also requires Src family kinase activities. Furthermore, tensin3 forms a complex with focal adhesion kinase and p130Cas in MDA-MB-468 cells. Addition of EGF to the cells induces dephosphorylation of these two molecules, leads to disassociation of the tensin3-focal adhesion kinase-p130Cas complex, and enhances the interaction between tensin3 and EGF receptor. Our results demonstrate that tensin3 may function as a platform for the disassembly of EGF-related signaling complexes at focal adhesions.

Original languageEnglish (US)
Pages (from-to)225-232
Number of pages8
JournalMolecular Cancer Research
Volume2
Issue number4
StatePublished - Apr 2004

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Epidermal Growth Factor
Tyrosine
Phosphorylation
Focal Adhesion Protein-Tyrosine Kinases
Epidermal Growth Factor Receptor
Phosphatidylinositol 3-Kinase
Focal Adhesions
src-Family Kinases
Actin Cytoskeleton
Endothelium
Amino Acid Sequence
Epithelium
Complementary DNA
Fibroblasts
Tensins
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

Epidermal growth factor modulates tyrosine phosphorylation of a novel tensin family member, tensin3. / Cui, Yumin; Liao, Yi Chun; Lo, Su Hao.

In: Molecular Cancer Research, Vol. 2, No. 4, 04.2004, p. 225-232.

Research output: Contribution to journalArticle

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