(-)-Epicatechin reduces blood pressure increase in high-fructose-fed rats: Effects on the determinants of nitric oxide bioavailability

Maria C. Litterio, Marcela A. Vazquez Prieto, Ana M. Adamo, Rosana Elesgaray, Patricia I. Oteiza, Monica Galleano, Cesar G. Fraga

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This work investigated the blood pressure (BP)-lowering effect of the flavanol (-)-epicatechin in a model of metabolic syndrome. Rats were fed a regular chow diet without (Control) or with 10% (w/v) fructose in the drinking water (high fructose, HF) for 8 weeks. A subgroup of the HF-fed rats was supplemented with (-)-epicatechin 20 mg/kg body weight (HF-EC). Dietary (-)-epicatechin reverted the increase in BP caused by the fructose treatment. In aorta, superoxide anion production and the expression of the NADPH oxidase (NOX) subunits p47phox and p22phox were enhanced in the HF-fed rats. The increase was prevented by (-)-epicatechin. Similar profile was observed for NOX4 expression. The activity of aorta nitric oxide synthase (NOS) was increased in the HF group and was even higher in the HF-EC rats. These effects were paralleled by increased endothelial NOS phosphorylation at the activation site Ser1177. Among the more relevant mitogen-activated protein kinase pathways in vascular tissue, c-Jun-N-terminal kinase was shown to be activated in the aorta of the HF-fed rats, and (-)-epicatechin supplementation mitigated this activation. Thus, the results suggest that dietary (-)-epicatechin supplementation prevented hypertension in HF-fed rats, decreasing superoxide anion production and elevating NOS activity, favoring an increase in NO bioavailability.

Original languageEnglish (US)
Pages (from-to)745-751
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume26
Issue number7
DOIs
StatePublished - 2015

Fingerprint

Catechin
Blood pressure
Fructose
Biological Availability
Rats
Nitric Oxide
Blood Pressure
Aorta
Nitric Oxide Synthase
Superoxides
Chemical activation
Phosphorylation
Pressure effects
JNK Mitogen-Activated Protein Kinases
Nitric Oxide Synthase Type III
NADPH Oxidase
Nutrition
Dietary Supplements
Mitogen-Activated Protein Kinases
Drinking Water

Keywords

  • Flavonoids
  • Fructose
  • Hypertension
  • Metabolic syndrome
  • Oxidants
  • Superoxide anion

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

(-)-Epicatechin reduces blood pressure increase in high-fructose-fed rats : Effects on the determinants of nitric oxide bioavailability. / Litterio, Maria C.; Vazquez Prieto, Marcela A.; Adamo, Ana M.; Elesgaray, Rosana; Oteiza, Patricia I.; Galleano, Monica; Fraga, Cesar G.

In: Journal of Nutritional Biochemistry, Vol. 26, No. 7, 2015, p. 745-751.

Research output: Contribution to journalArticle

Litterio, Maria C. ; Vazquez Prieto, Marcela A. ; Adamo, Ana M. ; Elesgaray, Rosana ; Oteiza, Patricia I. ; Galleano, Monica ; Fraga, Cesar G. / (-)-Epicatechin reduces blood pressure increase in high-fructose-fed rats : Effects on the determinants of nitric oxide bioavailability. In: Journal of Nutritional Biochemistry. 2015 ; Vol. 26, No. 7. pp. 745-751.
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AU - Elesgaray, Rosana

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AU - Galleano, Monica

AU - Fraga, Cesar G.

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