Abstract
Introduction: High plasma levels of sEPCR lead to dysfunction of the EPCR-mediated coagulation. We have evaluated the role of EPCR A3 haplotype with its representative promoter variant 1651 C-G in a total of twenty-seven pediatric stroke patients and fifty-nine healthy subjects. Materials and methods: Genotyping of the A3 haplotype was performed with RFLP analysis. Plasma sEPCR levels were measured with ELISA. The mutant 1651 G allele frequency was observed to be 0.166 in the patient group. Common risk factors such as FV 1691 G-A and PT 20210 G-A mutations were also screened. Results and conclusions: None of the patients with sEPCR levels below 100 ng/ml carried the A3 haplotype, while patients with elevated sEPCR levels carried the A3 haplotype either in a heterozygous or homozygous state. Our study confirms that there is a strong association between A3 haplotype and elevated sEPCR levels. We suggest that elevated sEPCR levels might increase the risk of stroke at pediatric age when compared to controls. Studies with large series of patients are warranted to confirm this hypothesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 47-52 |
| Number of pages | 6 |
| Journal | Thrombosis Research |
| Volume | 120 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2007 |
| Externally published | Yes |
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Keywords
- A3 haplotype
- EPCR gene
- Pediatric stroke
- sEPCR levels
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Hematology
Cite this
EPCR gene A3 haplotype and elevated soluble endothelial protein C receptor (sEPCR) levels in Turkish pediatric stroke patients. / Ulu, Arzu; Gunal, Denizay; Tiras, Serap; Egin, Yonca; Deda, Gülhis; Akar, Nejat.
In: Thrombosis Research, Vol. 120, No. 1, 2007, p. 47-52.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - EPCR gene A3 haplotype and elevated soluble endothelial protein C receptor (sEPCR) levels in Turkish pediatric stroke patients
AU - Ulu, Arzu
AU - Gunal, Denizay
AU - Tiras, Serap
AU - Egin, Yonca
AU - Deda, Gülhis
AU - Akar, Nejat
PY - 2007
Y1 - 2007
N2 - Introduction: High plasma levels of sEPCR lead to dysfunction of the EPCR-mediated coagulation. We have evaluated the role of EPCR A3 haplotype with its representative promoter variant 1651 C-G in a total of twenty-seven pediatric stroke patients and fifty-nine healthy subjects. Materials and methods: Genotyping of the A3 haplotype was performed with RFLP analysis. Plasma sEPCR levels were measured with ELISA. The mutant 1651 G allele frequency was observed to be 0.166 in the patient group. Common risk factors such as FV 1691 G-A and PT 20210 G-A mutations were also screened. Results and conclusions: None of the patients with sEPCR levels below 100 ng/ml carried the A3 haplotype, while patients with elevated sEPCR levels carried the A3 haplotype either in a heterozygous or homozygous state. Our study confirms that there is a strong association between A3 haplotype and elevated sEPCR levels. We suggest that elevated sEPCR levels might increase the risk of stroke at pediatric age when compared to controls. Studies with large series of patients are warranted to confirm this hypothesis.
AB - Introduction: High plasma levels of sEPCR lead to dysfunction of the EPCR-mediated coagulation. We have evaluated the role of EPCR A3 haplotype with its representative promoter variant 1651 C-G in a total of twenty-seven pediatric stroke patients and fifty-nine healthy subjects. Materials and methods: Genotyping of the A3 haplotype was performed with RFLP analysis. Plasma sEPCR levels were measured with ELISA. The mutant 1651 G allele frequency was observed to be 0.166 in the patient group. Common risk factors such as FV 1691 G-A and PT 20210 G-A mutations were also screened. Results and conclusions: None of the patients with sEPCR levels below 100 ng/ml carried the A3 haplotype, while patients with elevated sEPCR levels carried the A3 haplotype either in a heterozygous or homozygous state. Our study confirms that there is a strong association between A3 haplotype and elevated sEPCR levels. We suggest that elevated sEPCR levels might increase the risk of stroke at pediatric age when compared to controls. Studies with large series of patients are warranted to confirm this hypothesis.
KW - A3 haplotype
KW - EPCR gene
KW - Pediatric stroke
KW - sEPCR levels
UR - http://www.scopus.com/inward/record.url?scp=34247108701&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34247108701&partnerID=8YFLogxK
U2 - 10.1016/j.thromres.2006.08.004
DO - 10.1016/j.thromres.2006.08.004
M3 - Article
C2 - 17027065
AN - SCOPUS:34247108701
VL - 120
SP - 47
EP - 52
JO - Thrombosis Research
JF - Thrombosis Research
SN - 0049-3848
IS - 1
ER -