Enzyme-probe conjugates as analytical tools in diagnostics

Charles A. Dangler, Bennie Osburn

Research output: Contribution to journalConference article

2 Scopus citations

Abstract

Applications of enzymatic detection systems in the context of diagnostics are presented in this article. An overview of detection systems, including those based on non-enzymatic markers such as radioactive, fluorescent and particulate labels, is discussed initially. Direct and indirect strategies for applying detection systems are also presented with respect to the identification of immunoglobulin and nucleic acid probe molecules. Enzymatic detection systems are discussed with an emphasis on chromogenic enzyme reactions. Methods of designing enzymatic assays often involve using molecular bridges between enzyme molecules and the probe species; immunodetection is discussed in this context as well as procedures for attaching immunodetectable moieties to probe molecules. Finally, current and potential developments which may improve the application of enzymatic detection systems are briefly outlined.

Original languageEnglish (US)
Pages (from-to)230-241
Number of pages12
JournalACS Symposium Series
Issue number389
StatePublished - Dec 1 1989
Externally publishedYes
EventThird Chemical Congress of North America (195th National Meeting of the American Chemical Society) - Biocatalysis in Agricultural Biotechnology - Toronto, Ont, Can
Duration: Jun 5 1988Jun 11 1988

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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