Enzyme-Linked immunosorbent assay for the pyrethroid permethrin

Guomin Shan, Whitney R. Leeman, Donald W. Stoutamire, Shirley J. Gee, Daniel P Y Chang, Bruce D. Hammock

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Permethrin is a predominant pyrethroid widely used in agriculture and public health. A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of permethrin was developed. Two haptens, the trans- and cis-isomers of 3-(4-aminophenoxy)benzyl-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropanecarb oxylate, were synthesized and conjugated with thyroglobulin as immunogens. Four antisera were generated and screened against six different coating antigens. The resulting ELISA has an I50 value of 2.50/μg/L and relatively low cross-reactivities with other major pyrethroids, such as esfenvalerate, cypermethrin, deltamethrin, and cyfluthrin. Methanol was found to be the best solvent for this ELISA, with optimal sensitivity observed at a concentration of 40% (v/v). The assay parameters are unchanged at pH values between 5.0 and 8.0, whereas higher ionic strengths (>0.2 M PBS) strongly suppress the absorbances. River water samples fortified with permethrin were analyzed according to this method and validated by GC-MS. Good recoveries and correlation with spike levels were observed, suggesting this immunoassay is valuable for environmental monitoring and toxicological studies at parts per trillion levels of permethrin.

Original languageEnglish (US)
Pages (from-to)4032-4040
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume48
Issue number9
DOIs
StatePublished - 2000

Keywords

  • ELISA
  • Environmental monitoring
  • Permethrin
  • Pyrethroid
  • Toxicological studies

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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  • Cite this

    Shan, G., Leeman, W. R., Stoutamire, D. W., Gee, S. J., Chang, D. P. Y., & Hammock, B. D. (2000). Enzyme-Linked immunosorbent assay for the pyrethroid permethrin. Journal of Agricultural and Food Chemistry, 48(9), 4032-4040. https://doi.org/10.1021/jf000351x