Enzyme-linked immunosorbent assay for the detection of antibodies to bovine viral diarrhea virus in bovine sera

Chu Hsien-Jue Chu, Chung Zee Yuan Chung Zee, Alex Ardans, Dai Kang Dai

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27 Scopus citations

Abstract

A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was established for the detection of antibodies to bovine viral diarrhea virus (BVDV) in bovine sera. Polyethylene-glycol concentrated, equilibrium density gradient purified BVDV was used as test antigen at an optimal amount of 1 μg/well, whereas the optimal concentration of conjugate was at 1 2000 dilution. The standardized test encountered no non-specific reaction with test sera at a starting dilution of 1 10. A total of 50 bovine serum samples was assayed for the presence of antibodies against BVDV by ELISA and serum neutralization test (SNT). A positive correlation between the 2 tests was found. However, ELISA could be as much as 500-fold more sensitive than SNT in detecting low levels of BVDV antibodies.

Original languageEnglish (US)
Pages (from-to)325-333
Number of pages9
JournalVeterinary Microbiology
Volume10
Issue number4
DOIs
StatePublished - Jan 1 1985

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ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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