Enzyme-linked immunosorbent assay for the detection of antibodies to bovine virus diarrhea virus in sera from border disease virus-infected sheep.

H. J. Chu, M. M. Sawyer, C. A. Anderson, Robert Higgins, Y. C. Zee

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

An enzyme-linked immunosorbent assay (ELISA) was established for the rapid detection of specific antibodies against the causative agent of border disease in ovine sera. Polyethylene-glycol concentrated, equilibrium density gradient purified bovine virus diarrhea virus was used as test antigen. The optimal amount of antigen was 0.5 microgram/well, and the optimal concentration of conjugate was at 1/4,000 dilution. A total of 20 ovine serum samples, which had been collected from animals with or without border disease, were compared by ELISA and serum neutralization test for the detection of border disease-specific antibodies. ELISA was shown to be equally specific but less time-consuming and easier to perform than serum neutralization test. A positive correlation (r = 0.60) between the two tests was found.

Original languageEnglish (US)
Pages (from-to)281-283
Number of pages3
JournalCanadian journal of veterinary research = Revue canadienne de recherche veterinaire
Volume51
Issue number2
StatePublished - Apr 1 1987
Externally publishedYes

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'Enzyme-linked immunosorbent assay for the detection of antibodies to bovine virus diarrhea virus in sera from border disease virus-infected sheep.'. Together they form a unique fingerprint.

  • Cite this