Enzyme-linked immunosorbent assay for quantification of bacillus thuringiensis var. kurstaki crystalline protein in some commercial formulations

Kanth M S Sundaram, Alam Sundaram, Shirley J. Gee, Robert O. Harrison, Bruce D. Hammock

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

A sandwich type enzyme-linked immunosorbent assay (ELISA) has been developed to detect and quantify Bacillus thuringiensis var. kurstaki delta-endotoxin in nine commercial formulations that are being used for forest insect control in North America. Formulation ingredients have interfered with the toxin response in three of the nine formulation, and the total protein needed to be separated from formulants for ELISA. Moreover, total protein are consistently higher (about 40 to 60%) in all formulations than in delta-endotoxin content because of the presence of inactive proteins.

Original languageEnglish (US)
Title of host publicationASTM Special Technical Publication
EditorsFranklin R. Hall, Paul D. Berger, Herbert M. Collins
PublisherASTM
Pages297-312
Number of pages16
Edition1234
StatePublished - 1995
Externally publishedYes
EventProceedings of the 14th Symposium on Pesticide Formulations and Application Systems - Dallas, TX, USA
Duration: Oct 12 1995Oct 13 1995

Other

OtherProceedings of the 14th Symposium on Pesticide Formulations and Application Systems
CityDallas, TX, USA
Period10/12/9510/13/95

ASJC Scopus subject areas

  • Engineering(all)

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