Enzyme-linked immunosorbent assay for quantification of bacillus thuringiensis var. kurstaki crystalline protein in some commercial formulations

Kanth M S Sundaram, Alam Sundaram, Shirley J. Gee, Robert O. Harrison, Bruce D. Hammock

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

A sandwich type enzyme-linked immunosorbent assay (ELISA) has been developed to detect and quantify Bacillus thuringiensis var. kurstaki delta-endotoxin in nine commercial formulations that are being used for forest insect control in North America. Formulation ingredients have interfered with the toxin response in three of the nine formulation, and the total protein needed to be separated from formulants for ELISA. Moreover, total protein are consistently higher (about 40 to 60%) in all formulations than in delta-endotoxin content because of the presence of inactive proteins.

Original languageEnglish (US)
Title of host publicationASTM Special Technical Publication
EditorsFranklin R. Hall, Paul D. Berger, Herbert M. Collins
PublisherASTM
Pages297-312
Number of pages16
Edition1234
StatePublished - 1995
Externally publishedYes
EventProceedings of the 14th Symposium on Pesticide Formulations and Application Systems - Dallas, TX, USA
Duration: Oct 12 1995Oct 13 1995

Other

OtherProceedings of the 14th Symposium on Pesticide Formulations and Application Systems
CityDallas, TX, USA
Period10/12/9510/13/95

Fingerprint

Bacilli
Assays
Enzymes
Crystalline materials
Proteins
Insect control
Immunosorbents

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Sundaram, K. M. S., Sundaram, A., Gee, S. J., Harrison, R. O., & Hammock, B. D. (1995). Enzyme-linked immunosorbent assay for quantification of bacillus thuringiensis var. kurstaki crystalline protein in some commercial formulations. In F. R. Hall, P. D. Berger, & H. M. Collins (Eds.), ASTM Special Technical Publication (1234 ed., pp. 297-312). ASTM.

Enzyme-linked immunosorbent assay for quantification of bacillus thuringiensis var. kurstaki crystalline protein in some commercial formulations. / Sundaram, Kanth M S; Sundaram, Alam; Gee, Shirley J.; Harrison, Robert O.; Hammock, Bruce D.

ASTM Special Technical Publication. ed. / Franklin R. Hall; Paul D. Berger; Herbert M. Collins. 1234. ed. ASTM, 1995. p. 297-312.

Research output: Chapter in Book/Report/Conference proceedingChapter

Sundaram, KMS, Sundaram, A, Gee, SJ, Harrison, RO & Hammock, BD 1995, Enzyme-linked immunosorbent assay for quantification of bacillus thuringiensis var. kurstaki crystalline protein in some commercial formulations. in FR Hall, PD Berger & HM Collins (eds), ASTM Special Technical Publication. 1234 edn, ASTM, pp. 297-312, Proceedings of the 14th Symposium on Pesticide Formulations and Application Systems, Dallas, TX, USA, 10/12/95.
Sundaram KMS, Sundaram A, Gee SJ, Harrison RO, Hammock BD. Enzyme-linked immunosorbent assay for quantification of bacillus thuringiensis var. kurstaki crystalline protein in some commercial formulations. In Hall FR, Berger PD, Collins HM, editors, ASTM Special Technical Publication. 1234 ed. ASTM. 1995. p. 297-312
Sundaram, Kanth M S ; Sundaram, Alam ; Gee, Shirley J. ; Harrison, Robert O. ; Hammock, Bruce D. / Enzyme-linked immunosorbent assay for quantification of bacillus thuringiensis var. kurstaki crystalline protein in some commercial formulations. ASTM Special Technical Publication. editor / Franklin R. Hall ; Paul D. Berger ; Herbert M. Collins. 1234. ed. ASTM, 1995. pp. 297-312
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