Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed for the determination of paraquat in human exposure samples. Using an antibody dilution of 1/5000, concentrations of paraquat cation in the range 0.1-27 ng/mL could be measured. Limit of detection ranged from 0.1 to 1.0 ng/mL depending on the matrix analyzed. The method had good precision, with less than 5% between-run and less than 4% within-run variation, and was selective for paraquat showing minimal cross reactivity with ethylparaquat, diquat, and other compounds. In comparison with a gas chromatographic method, the ELISA gave higher recoveries, was less labor intensive, and was more sensitive. The ELISA was applied to paraquat in high volume glass fiber filters, personal air monitors, worker clothing patches, and hand washes collected during aerial spraying of cotton. When the same filters were analyzed by both ELISA and GC, the ELISA consistently resulted in higher values in keeping with the greater efficiency of the sample preparation steps of ELISA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1866-1873 |
| Number of pages | 8 |
| Journal | Analytical Chemistry |
| Volume | 58 |
| Issue number | 8 |
| State | Published - 1986 |
Fingerprint
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Enzyme-linked immunosorbent assay for paraquat and its application to exposure analysis. / Van Emon, Jeanette; Hammock, Bruce; Seiber, James N.
In: Analytical Chemistry, Vol. 58, No. 8, 1986, p. 1866-1873.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enzyme-linked immunosorbent assay for paraquat and its application to exposure analysis
AU - Van Emon, Jeanette
AU - Hammock, Bruce
AU - Seiber, James N.
PY - 1986
Y1 - 1986
N2 - An enzyme-linked immunosorbent assay (ELISA) was developed for the determination of paraquat in human exposure samples. Using an antibody dilution of 1/5000, concentrations of paraquat cation in the range 0.1-27 ng/mL could be measured. Limit of detection ranged from 0.1 to 1.0 ng/mL depending on the matrix analyzed. The method had good precision, with less than 5% between-run and less than 4% within-run variation, and was selective for paraquat showing minimal cross reactivity with ethylparaquat, diquat, and other compounds. In comparison with a gas chromatographic method, the ELISA gave higher recoveries, was less labor intensive, and was more sensitive. The ELISA was applied to paraquat in high volume glass fiber filters, personal air monitors, worker clothing patches, and hand washes collected during aerial spraying of cotton. When the same filters were analyzed by both ELISA and GC, the ELISA consistently resulted in higher values in keeping with the greater efficiency of the sample preparation steps of ELISA.
AB - An enzyme-linked immunosorbent assay (ELISA) was developed for the determination of paraquat in human exposure samples. Using an antibody dilution of 1/5000, concentrations of paraquat cation in the range 0.1-27 ng/mL could be measured. Limit of detection ranged from 0.1 to 1.0 ng/mL depending on the matrix analyzed. The method had good precision, with less than 5% between-run and less than 4% within-run variation, and was selective for paraquat showing minimal cross reactivity with ethylparaquat, diquat, and other compounds. In comparison with a gas chromatographic method, the ELISA gave higher recoveries, was less labor intensive, and was more sensitive. The ELISA was applied to paraquat in high volume glass fiber filters, personal air monitors, worker clothing patches, and hand washes collected during aerial spraying of cotton. When the same filters were analyzed by both ELISA and GC, the ELISA consistently resulted in higher values in keeping with the greater efficiency of the sample preparation steps of ELISA.
UR - http://www.scopus.com/inward/record.url?scp=0022642294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022642294&partnerID=8YFLogxK
M3 - Article
C2 - 3752512
AN - SCOPUS:0022642294
VL - 58
SP - 1866
EP - 1873
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 8
ER -