Enzyme and size profiles in chronically inactive cat soleus muscle fibers

S. C. Graham, R. R. Roy, C. Navarro, B. Jiang, D. Pierotti, S. Bodine-Fowler, V. R. Edgerton

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Spinal isolation (SI), i.e., the isolation of the lumbar spinal cord via a rostral and a caudal cord transection and bilateral dorsal rhizotomy, was used to determine the effects of chronic (6 months) inactivity on the size and metabolic properties of fibers in the cat soleus. Fibers were classified as dark or light, based on their staining reactions to myosin ATPase, alkaline preincubation, and immunohistochemically as expressing fast and/or slow myosin heavy chains (MHC). Succinate dehydrogenase (SDH) and α- glycerophosphate dehydrogenase (GPD) activities were assessed histochemically. Following SI, both the light and the dark ATPase fibers in the SI cats were significantly smaller than the light ATPase fibers in the controls. Normally 100% of the fibers were light ATPase and reacted exclusively with the slow antibody. After SI, ~45% of the fibers were dark ATPase fibers, many reacting with both fast and slow MHC antibodies. The total amount and concentration of GPD were higher in the light and dark ATPase fibers in SI compared with light ATPase fibers in controls. In contrast, although the total amount of SDH per fiber was decreased, reflecting the decrease in fiber size, the mean SDH concentration per fiber was unchanged following SI. These data indicate that there is a close coordination in the regulation of GPD activity and the type of myosin. SDH activity, on the other hand, appears to be resistant to decreased levels of activity and unloading, i.e., there seems to be a minimum level of oxidative potential in the soleus that is independent of activity level. Fiber sizes, however, are very sensitive to less-than-normal amounts of neuromuscular activity and/or loading.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalMuscle and Nerve
Volume15
Issue number1
StatePublished - 1992

Fingerprint

Adenosine Triphosphatases
Skeletal Muscle
Cats
Succinate Dehydrogenase
Glycerolphosphate Dehydrogenase
Light
Enzymes
Myosin Heavy Chains
Myosins
Spinal Cord
Rhizotomy
Antibodies
Staining and Labeling

Keywords

  • disuse
  • fiber types
  • glycolytic potential
  • myosin heavy chains
  • oxidative potential

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Graham, S. C., Roy, R. R., Navarro, C., Jiang, B., Pierotti, D., Bodine-Fowler, S., & Edgerton, V. R. (1992). Enzyme and size profiles in chronically inactive cat soleus muscle fibers. Muscle and Nerve, 15(1), 27-36.

Enzyme and size profiles in chronically inactive cat soleus muscle fibers. / Graham, S. C.; Roy, R. R.; Navarro, C.; Jiang, B.; Pierotti, D.; Bodine-Fowler, S.; Edgerton, V. R.

In: Muscle and Nerve, Vol. 15, No. 1, 1992, p. 27-36.

Research output: Contribution to journalArticle

Graham, SC, Roy, RR, Navarro, C, Jiang, B, Pierotti, D, Bodine-Fowler, S & Edgerton, VR 1992, 'Enzyme and size profiles in chronically inactive cat soleus muscle fibers', Muscle and Nerve, vol. 15, no. 1, pp. 27-36.
Graham SC, Roy RR, Navarro C, Jiang B, Pierotti D, Bodine-Fowler S et al. Enzyme and size profiles in chronically inactive cat soleus muscle fibers. Muscle and Nerve. 1992;15(1):27-36.
Graham, S. C. ; Roy, R. R. ; Navarro, C. ; Jiang, B. ; Pierotti, D. ; Bodine-Fowler, S. ; Edgerton, V. R. / Enzyme and size profiles in chronically inactive cat soleus muscle fibers. In: Muscle and Nerve. 1992 ; Vol. 15, No. 1. pp. 27-36.
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AU - Roy, R. R.

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AU - Bodine-Fowler, S.

AU - Edgerton, V. R.

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