Enzymatic synthesis of human blood group P1 pentasaccharide antigen

Dawa Tsering, Congcong Chen, Jinfeng Ye, Zhipeng Han, Bai qian Jing, Xian wei Liu, Xi Chen, Fengshan Wang, Peixue Ling, Hongzhi Cao

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The enzymatic synthesis of biologically important and structurally unique human P1PK blood group type P1 pentasaccharide antigen is described. This synthesis features a three-step sequential one-pot multienzyme (OPME) glycosylation for the stepwise enzymatic chain elongation of readily available lactoside acceptor with cheap and commercially available galactose and N-acetylglucosamine as donor precursors. This enzymatic synthesis provides an operationally simple approach to access P1 pentasaccharide and its structurally related Gb3 and P1 trisaccharide epitopes.

Original languageEnglish (US)
Pages (from-to)39-43
Number of pages5
JournalCarbohydrate Research
Volume438
DOIs
StatePublished - Jan 13 2017

Keywords

  • Glycosylation
  • Glycosyltransferase
  • Human blood group
  • One-pot multienzyme
  • P1 antigen

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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  • Cite this

    Tsering, D., Chen, C., Ye, J., Han, Z., Jing, B. Q., Liu, X. W., Chen, X., Wang, F., Ling, P., & Cao, H. (2017). Enzymatic synthesis of human blood group P1 pentasaccharide antigen. Carbohydrate Research, 438, 39-43. https://doi.org/10.1016/j.carres.2016.11.019