Abstract
The cell wall of the spherule phase of Coccidioides immitis was studied through the use of degradative enzymes and transmission electron microscopy. The evidence obtained indicated that the cell wall has multiple layers of differing chemical composition. In the mature cell wall, the inner half, adjacent to the cytoplasmic membrane, appeared to be a matrix of chitin and β-(1-3)-glucan. In contrast, the outer half of the cell wall was found to be largely α-(1-3)-glucan. Interspersed throughout the wall was what appeared to be a mannan-protein complex. This complex had the effect of masking the multiple layers of the wall, and appeared to be responsible for holding the layers together and anchoring the wall to the membrane. The evidence suggested a sequential addition of wall components to maturing cleavage planes of the endosporulating spherule.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 136-152 |
| Number of pages | 17 |
| Journal | Experimental Mycology |
| Volume | 6 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1982 |
Fingerprint
Keywords
- cell wall
- Coccidioides immitis
- enzymatic degradation
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
Cite this
Enzymatic degradation of the walls of spherules of Coccidioides immitis. / Hector, Richard F.; Pappagianis, Demosthenes.
In: Experimental Mycology, Vol. 6, No. 2, 1982, p. 136-152.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enzymatic degradation of the walls of spherules of Coccidioides immitis
AU - Hector, Richard F.
AU - Pappagianis, Demosthenes
PY - 1982
Y1 - 1982
N2 - The cell wall of the spherule phase of Coccidioides immitis was studied through the use of degradative enzymes and transmission electron microscopy. The evidence obtained indicated that the cell wall has multiple layers of differing chemical composition. In the mature cell wall, the inner half, adjacent to the cytoplasmic membrane, appeared to be a matrix of chitin and β-(1-3)-glucan. In contrast, the outer half of the cell wall was found to be largely α-(1-3)-glucan. Interspersed throughout the wall was what appeared to be a mannan-protein complex. This complex had the effect of masking the multiple layers of the wall, and appeared to be responsible for holding the layers together and anchoring the wall to the membrane. The evidence suggested a sequential addition of wall components to maturing cleavage planes of the endosporulating spherule.
AB - The cell wall of the spherule phase of Coccidioides immitis was studied through the use of degradative enzymes and transmission electron microscopy. The evidence obtained indicated that the cell wall has multiple layers of differing chemical composition. In the mature cell wall, the inner half, adjacent to the cytoplasmic membrane, appeared to be a matrix of chitin and β-(1-3)-glucan. In contrast, the outer half of the cell wall was found to be largely α-(1-3)-glucan. Interspersed throughout the wall was what appeared to be a mannan-protein complex. This complex had the effect of masking the multiple layers of the wall, and appeared to be responsible for holding the layers together and anchoring the wall to the membrane. The evidence suggested a sequential addition of wall components to maturing cleavage planes of the endosporulating spherule.
KW - cell wall
KW - Coccidioides immitis
KW - enzymatic degradation
UR - http://www.scopus.com/inward/record.url?scp=0020327828&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020327828&partnerID=8YFLogxK
U2 - 10.1016/0147-5975(82)90088-3
DO - 10.1016/0147-5975(82)90088-3
M3 - Article
AN - SCOPUS:0020327828
VL - 6
SP - 136
EP - 152
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
SN - 1087-1845
IS - 2
ER -