Environmentally relevant concentrations of herbicides impact non-target species at multiple sublethal endpoints

Simone Hasenbein, Jade Peralta, Sharon P. Lawler, Richard E Connon

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

There is concern over herbicide exposure effects on aquatic primary production and zooplankton as herbicides are found in aquatic ecosystems at concentrations potentially toxic to phytoplankton. We first aimed to determine the effect concentrations (growth inhibition) and mixture interactions of the herbicides diuron (0.5 to 50 μg/L) and hexazinone (0.5 to 40 μg/L) on the green algae Pseudokirchneriella subcapitata. Secondly, we evaluated chronic effects on Daphnia magna that were periodically fed on P. subcapitata that had been exposed to low, medium, and high concentrations. We hypothesized that based on the mode of action of the herbicides we would observe additive growth inhibition in algae, and sublethal effects on D. magna. Growth inhibition in P. subcapitata following mixture exposure was most consistent with the concentration addition (CA) concept; while the independent action (IA) model underestimated the combined effect. The lowest observed effect concentrations (LOEC) were 1.50 μg/L hexazinone, 1.18 μg/L diuron, and 0.125 TU (0.30 μg/L diuron × 0.12 μg/L hexazinone) in the single and binary mixture exposures, respectively. High hexazinone exposure decreased D. magna survival (80% vs. 55.6%). Neonate number was reduced by 13.9% in high mixture and 23.5% in high hexazinone treatments. Gravid body length was reduced by 9.5% following exposure to the high mixture. Herbicide exposure decreased neonate size, especially in later broods. Herbicides decreased the phototaxic responses of neonates in most treatments. Herbicide exposure effects were detected at environmentally relevant concentrations, levels considered to be safe according to current USEPA aquatic life benchmarks, suggesting that these benchmarks need to be updated to improve ecological risk assessment. As herbicides are some of the most applied pesticides worldwide, sublethal endpoints can serve as sensitive early warning tools to indicate their presence and can support regulatory assessments and monitoring to protect aquatic life.

Original languageEnglish (US)
Pages (from-to)733-743
Number of pages11
JournalScience of the Total Environment
Volume607-608
DOIs
StatePublished - Dec 31 2017

Fingerprint

hexazinone
Herbicides
herbicide
neonate
Diuron
diuron
Algae
Aquatic ecosystems
sublethal effect
Phytoplankton
Poisons
Pesticides
Binary mixtures
exposure
green alga
Risk assessment
aquatic ecosystem
effect
primary production
zooplankton

Keywords

  • Behavior
  • Cladocera
  • Maternal exposure
  • Mixture toxicity
  • Reproduction

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

Cite this

Environmentally relevant concentrations of herbicides impact non-target species at multiple sublethal endpoints. / Hasenbein, Simone; Peralta, Jade; Lawler, Sharon P.; Connon, Richard E.

In: Science of the Total Environment, Vol. 607-608, 31.12.2017, p. 733-743.

Research output: Contribution to journalArticle

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abstract = "There is concern over herbicide exposure effects on aquatic primary production and zooplankton as herbicides are found in aquatic ecosystems at concentrations potentially toxic to phytoplankton. We first aimed to determine the effect concentrations (growth inhibition) and mixture interactions of the herbicides diuron (0.5 to 50 μg/L) and hexazinone (0.5 to 40 μg/L) on the green algae Pseudokirchneriella subcapitata. Secondly, we evaluated chronic effects on Daphnia magna that were periodically fed on P. subcapitata that had been exposed to low, medium, and high concentrations. We hypothesized that based on the mode of action of the herbicides we would observe additive growth inhibition in algae, and sublethal effects on D. magna. Growth inhibition in P. subcapitata following mixture exposure was most consistent with the concentration addition (CA) concept; while the independent action (IA) model underestimated the combined effect. The lowest observed effect concentrations (LOEC) were 1.50 μg/L hexazinone, 1.18 μg/L diuron, and 0.125 TU (0.30 μg/L diuron × 0.12 μg/L hexazinone) in the single and binary mixture exposures, respectively. High hexazinone exposure decreased D. magna survival (80{\%} vs. 55.6{\%}). Neonate number was reduced by 13.9{\%} in high mixture and 23.5{\%} in high hexazinone treatments. Gravid body length was reduced by 9.5{\%} following exposure to the high mixture. Herbicide exposure decreased neonate size, especially in later broods. Herbicides decreased the phototaxic responses of neonates in most treatments. Herbicide exposure effects were detected at environmentally relevant concentrations, levels considered to be safe according to current USEPA aquatic life benchmarks, suggesting that these benchmarks need to be updated to improve ecological risk assessment. As herbicides are some of the most applied pesticides worldwide, sublethal endpoints can serve as sensitive early warning tools to indicate their presence and can support regulatory assessments and monitoring to protect aquatic life.",
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