Environmental survey for Listeria species in frozen milk product plants in California

Richard L. Walker, Leon H. Jensen, Hailu Kinde, Amy V. Alexander, Linda S. Owens

Research output: Contribution to journalReview article

30 Citations (Scopus)

Abstract

A survey of frozen milk product (FMP) plants in California was conducted to determine prevalence of Listeria spp. among plants and within specific areas in plants. Association of possible factors contributing to the presence of Listeria was analyzed. Of 922 samples, 111 (12%) were positive for Listeria spp. Listeria monocytogenes and L. innocua were the only species isolated. Of 39 plants sampled, L. monocytogenes was the only species recovered from 5 (12.8%) plants and L. innocua was the only species recovered from 13 (33.3%) plants. Both species were isolated from 9 (23.1%) plants. No Listeria was isolated from 12 (30.8%) plants. A minimum of five colonies was identified from each positive site. Only one species was identified from 105 of the 111 positive sites (94.6%). There was a statistically significant association of culture results from selected sites in plants with corresponding drain cultures; however, from 9 of 106 (8.5%) site/drain combinations no Listeria was isolated from the drain when the selected sites were positive. Type of product received and type of pasteurization did not influence recovery of Listeria from a plant. There was a significant association with size of the plant and recovery of Listeria. Level of sanitation and extent of environmental contamination control program (ECCP), individually, were not associated with recovery of Listeria from a plant. However, the rate of recovery of Listeria from plants with above average sanitation and excellent or moderate ECCP (N=7) was 6.81%, whereas the recovery rate from plants with below average sanitation and slight or no ECCP (N=7) was 27.5%. The highest recovery rates of Listeria in FMP plants were in batch flavoring, freezing and ingredient blending, and packaging filling areas.

Original languageEnglish (US)
Pages (from-to)178-182
Number of pages5
JournalJournal of Food Protection
Volume54
Issue number3
StatePublished - Mar 1 1991
Externally publishedYes

Fingerprint

frozen milk
Listeria
dairy products
Milk
Sanitation
sanitation
pollution
Surveys and Questionnaires
Listeria monocytogenes
Pasteurization

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Walker, R. L., Jensen, L. H., Kinde, H., Alexander, A. V., & Owens, L. S. (1991). Environmental survey for Listeria species in frozen milk product plants in California. Journal of Food Protection, 54(3), 178-182.

Environmental survey for Listeria species in frozen milk product plants in California. / Walker, Richard L.; Jensen, Leon H.; Kinde, Hailu; Alexander, Amy V.; Owens, Linda S.

In: Journal of Food Protection, Vol. 54, No. 3, 01.03.1991, p. 178-182.

Research output: Contribution to journalReview article

Walker, RL, Jensen, LH, Kinde, H, Alexander, AV & Owens, LS 1991, 'Environmental survey for Listeria species in frozen milk product plants in California', Journal of Food Protection, vol. 54, no. 3, pp. 178-182.
Walker, Richard L. ; Jensen, Leon H. ; Kinde, Hailu ; Alexander, Amy V. ; Owens, Linda S. / Environmental survey for Listeria species in frozen milk product plants in California. In: Journal of Food Protection. 1991 ; Vol. 54, No. 3. pp. 178-182.
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abstract = "A survey of frozen milk product (FMP) plants in California was conducted to determine prevalence of Listeria spp. among plants and within specific areas in plants. Association of possible factors contributing to the presence of Listeria was analyzed. Of 922 samples, 111 (12{\%}) were positive for Listeria spp. Listeria monocytogenes and L. innocua were the only species isolated. Of 39 plants sampled, L. monocytogenes was the only species recovered from 5 (12.8{\%}) plants and L. innocua was the only species recovered from 13 (33.3{\%}) plants. Both species were isolated from 9 (23.1{\%}) plants. No Listeria was isolated from 12 (30.8{\%}) plants. A minimum of five colonies was identified from each positive site. Only one species was identified from 105 of the 111 positive sites (94.6{\%}). There was a statistically significant association of culture results from selected sites in plants with corresponding drain cultures; however, from 9 of 106 (8.5{\%}) site/drain combinations no Listeria was isolated from the drain when the selected sites were positive. Type of product received and type of pasteurization did not influence recovery of Listeria from a plant. There was a significant association with size of the plant and recovery of Listeria. Level of sanitation and extent of environmental contamination control program (ECCP), individually, were not associated with recovery of Listeria from a plant. However, the rate of recovery of Listeria from plants with above average sanitation and excellent or moderate ECCP (N=7) was 6.81{\%}, whereas the recovery rate from plants with below average sanitation and slight or no ECCP (N=7) was 27.5{\%}. The highest recovery rates of Listeria in FMP plants were in batch flavoring, freezing and ingredient blending, and packaging filling areas.",
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