Environmental persistence and disinfectant susceptibility of Klebsiella pneumoniae recovered from pinnipeds stranded on the California Coast

Esteban Soto, Samah M.R. Abdelrazek, Carl Basbas, Pádraig J. Duignan, Carlos Rios, Barbara A. Byrne

Research output: Contribution to journalArticle

Abstract

Hypermucoviscous K. pneumoniae (HMV) are emergent zoonotic pathogens associated with increased invasiveness and pathogenicity in terrestrial and marine mammals. In this study, HMV and non-HMV isolates recovered from stranded pinnipeds were used to investigate: 1) their persistence in sea and fresh water microcosms at 10 and 20°C, 2) their capacity to form biofilms, and 3) the biocide efficacy of four disinfectants on their planktonic and biofilm phenotypes. Results indicated that although HMV isolates were significantly more mucoviscous, non-HMV isolates displayed significantly greater capacity to form biofilms (p < 0.05). Additionally, non-HMV isolates persisted in greater numbers in both sea- and freshwater, particularly at 20°C. These two phenomena could be associated with the greater growth observed for non-HMV isolates in in-vitro growth-curve assays (p < 0.05). Similar susceptibility to disinfectants was detected in HMV and non-HMV isolates when exposed for 24 h; however, the minimal biofilm disinfectant eradication concentration for HMV isolates was significantly higher than that for non-HMV when exposed to disinfectants for 0.5 h. This information should be taken into consideration when developing biosecurity protocols in facilities holding marine mammals in captivity.

Original languageEnglish (US)
Article number108554
JournalVeterinary Microbiology
Volume241
DOIs
StatePublished - Feb 2020

Fingerprint

Pinnipedia
Disinfectants
Klebsiella pneumoniae
disinfectants
biofilm
Biofilms
coasts
marine mammals
Seawater
Fresh Water
seawater
Mammals
biocides
biosecurity
Zoonoses
Growth
pathogenicity
Virulence
phenotype
pathogens

Keywords

  • Biofilm
  • Disinfectant
  • Emergent
  • Hypermucoviscous
  • Klebsiella
  • Microcosoms

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Environmental persistence and disinfectant susceptibility of Klebsiella pneumoniae recovered from pinnipeds stranded on the California Coast. / Soto, Esteban; Abdelrazek, Samah M.R.; Basbas, Carl; Duignan, Pádraig J.; Rios, Carlos; Byrne, Barbara A.

In: Veterinary Microbiology, Vol. 241, 108554, 02.2020.

Research output: Contribution to journalArticle

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