Environmental Genome Shotgun Sequencing of the Sargasso Sea

J. Craig Venter, Karin Remington, John F. Heidelberg, Aaron L. Halpern, Doug Rusch, Jonathan A Eisen, Dongying Wu, Ian Paulsen, Karen E. Nelson, William Nelson, Derrick E. Fouts, Samuel Levy, Anthony H. Knap, Michael W. Lomas, Ken Nealson, Owen White, Jeremy Peterson, Jeff Hoffman, Rachel Parsons, Holly Baden-TillsonCynthia Pfannkoch, Yu Hui Rogers, Hamilton O. Smith

Research output: Contribution to journalArticle

2721 Citations (Scopus)

Abstract

We have applied "whole-genome shotgun sequencing" to microbial populations collected en masse on tangential flow and impact filters from seawater samples collected from the Sargasso Sea near Bermuda. A total of 1.045 billion base pairs of nonredundant sequence was generated, annotated, and analyzed to elucidate the gene content, diversity, and relative abundance of the organisms within these environmental samples. These data are estimated to derive from at least 1800 genomic species based on sequence relatedness, including 148 previously unknown bacterial phylotypes. We have identified over 1.2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-tike photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity.

Original languageEnglish (US)
Pages (from-to)66-74
Number of pages9
JournalScience
Volume304
Issue number5667
DOIs
StatePublished - Apr 2 2004
Externally publishedYes

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Firearms
Oceans and Seas
Bermuda
Genome
Rhodopsin
Seawater
Base Pairing
Genes
Population

ASJC Scopus subject areas

  • General

Cite this

Venter, J. C., Remington, K., Heidelberg, J. F., Halpern, A. L., Rusch, D., Eisen, J. A., ... Smith, H. O. (2004). Environmental Genome Shotgun Sequencing of the Sargasso Sea. Science, 304(5667), 66-74. https://doi.org/10.1126/science.1093857

Environmental Genome Shotgun Sequencing of the Sargasso Sea. / Venter, J. Craig; Remington, Karin; Heidelberg, John F.; Halpern, Aaron L.; Rusch, Doug; Eisen, Jonathan A; Wu, Dongying; Paulsen, Ian; Nelson, Karen E.; Nelson, William; Fouts, Derrick E.; Levy, Samuel; Knap, Anthony H.; Lomas, Michael W.; Nealson, Ken; White, Owen; Peterson, Jeremy; Hoffman, Jeff; Parsons, Rachel; Baden-Tillson, Holly; Pfannkoch, Cynthia; Rogers, Yu Hui; Smith, Hamilton O.

In: Science, Vol. 304, No. 5667, 02.04.2004, p. 66-74.

Research output: Contribution to journalArticle

Venter, JC, Remington, K, Heidelberg, JF, Halpern, AL, Rusch, D, Eisen, JA, Wu, D, Paulsen, I, Nelson, KE, Nelson, W, Fouts, DE, Levy, S, Knap, AH, Lomas, MW, Nealson, K, White, O, Peterson, J, Hoffman, J, Parsons, R, Baden-Tillson, H, Pfannkoch, C, Rogers, YH & Smith, HO 2004, 'Environmental Genome Shotgun Sequencing of the Sargasso Sea', Science, vol. 304, no. 5667, pp. 66-74. https://doi.org/10.1126/science.1093857
Venter JC, Remington K, Heidelberg JF, Halpern AL, Rusch D, Eisen JA et al. Environmental Genome Shotgun Sequencing of the Sargasso Sea. Science. 2004 Apr 2;304(5667):66-74. https://doi.org/10.1126/science.1093857
Venter, J. Craig ; Remington, Karin ; Heidelberg, John F. ; Halpern, Aaron L. ; Rusch, Doug ; Eisen, Jonathan A ; Wu, Dongying ; Paulsen, Ian ; Nelson, Karen E. ; Nelson, William ; Fouts, Derrick E. ; Levy, Samuel ; Knap, Anthony H. ; Lomas, Michael W. ; Nealson, Ken ; White, Owen ; Peterson, Jeremy ; Hoffman, Jeff ; Parsons, Rachel ; Baden-Tillson, Holly ; Pfannkoch, Cynthia ; Rogers, Yu Hui ; Smith, Hamilton O. / Environmental Genome Shotgun Sequencing of the Sargasso Sea. In: Science. 2004 ; Vol. 304, No. 5667. pp. 66-74.
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