Environmental air sampling to detect exotic Newcastle disease virus in two California commercial poultry flocks

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The 2002-2003 Exotic Newcastle Disease (END) outbreak in Southern California poultry provided an opportunity to evaluate environmental air sampling as an efficient and cost-effective means of sampling flocks for detection of a circulating virus. Exotic Newcastle Disease virus was detected by real-time reverse transcriptase PCR from air samples collected using a wetted-wall cyclone-style air sampler placed within 2 m of birds in 2 commercial flocks suspected of being naturally exposed to END virus during the outbreak. Exotic Newcastle Disease virus was detected after 2 hours of air sampling the poultry-house environments of the 2 naturally infected flocks.

Original languageEnglish (US)
Pages (from-to)198-200
Number of pages3
JournalJournal of Veterinary Diagnostic Investigation
Volume17
Issue number2
StatePublished - Mar 2005

Fingerprint

Newcastle disease virus
Newcastle disease
Poultry
flocks
poultry
Air
air
Disease Outbreaks
Newcastle Disease
Cyclonic Storms
sampling
poultry housing
samplers
Reverse Transcriptase Polymerase Chain Reaction
Birds
Real-Time Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction
Viruses
Costs and Cost Analysis
viruses

Keywords

  • Air sampler
  • Environmental sampling
  • Exotic Newcastle Disease Virus
  • RT-PCR

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

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abstract = "The 2002-2003 Exotic Newcastle Disease (END) outbreak in Southern California poultry provided an opportunity to evaluate environmental air sampling as an efficient and cost-effective means of sampling flocks for detection of a circulating virus. Exotic Newcastle Disease virus was detected by real-time reverse transcriptase PCR from air samples collected using a wetted-wall cyclone-style air sampler placed within 2 m of birds in 2 commercial flocks suspected of being naturally exposed to END virus during the outbreak. Exotic Newcastle Disease virus was detected after 2 hours of air sampling the poultry-house environments of the 2 naturally infected flocks.",
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AU - Crossley, Beate

AU - Kinde, Hailu

AU - Ardans, Alex

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