Entrapment of recent thymic emigrants in lymphoid tissues from HIV-infected patients

Association with HIV cellular viral load

Mostafa A. Nokta, Xiao Dong Li, Lena Al-Harthi, Joan Nichols, Anna Pou, David Asmuth, Alan Landay, Richard B Pollard

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective(s): Depletion of thymus derived naive T-cells is a feature of HIV infection. Here the impact of HIV infection on the compartmentalization of recent thymic emigrants of (RTE) and naive T-cells was examined. Methods: Peripheral blood mononuclear cells (PBMC) and lymphoid tissue (LT) from 43 HIV-infected patients and 12 controls were examined for RTE distribution by measuring coding joint T-cell receptor excisional circles (cjTREC) by PCR and naive and memory T-cell subsets and adhesion molecules (L-selection, LFA-1) by flow cytometry. Results: In HIV-infected patients, the RTE as quantified by cjTRECs in CD4 LT cells were significantly higher than in PBMC. Their values, however, were less than in control subjects, in both the LT and PBMC compartments. This was associated with an increase in L-selectin and LFA-1 expression on LT derived T cells. In PBMC, a significant positive relationship between TREC and naive CD4 cells and an inverse relationship between TREC and cellular viral load (CVL) was observed. Whereas in LT, there was a positive relationship between cjTREC and both naive CD4 cell percentage and CVL. Conclusions: Collectively, the data suggests that LT is a significant reservoir for RTE. The RTE appeared to be entrapped in LT from HIV-infected subjects. Such entrapment is probably a response to the high viral load in these tissues. These observations may partially explain the decline in RTE observed in the peripheral blood of HIV-infected patients, and the delay in recovery of naive cells in blood after initiation of HAART.

Original languageEnglish (US)
Pages (from-to)2119-2127
Number of pages9
JournalAIDS
Volume16
Issue number16
DOIs
StatePublished - Nov 8 2002
Externally publishedYes

Fingerprint

Lymphoid Tissue
Viral Load
HIV
Blood Cells
Lymphocyte Function-Associated Antigen-1
T-Cell Antigen Receptor
T-Lymphocytes
HIV Infections
Joints
L-Selectin
Highly Active Antiretroviral Therapy
Cell Adhesion Molecules
T-Lymphocyte Subsets
Thymus Gland
Flow Cytometry
Lymphocytes
Polymerase Chain Reaction

Keywords

  • AIDS
  • HIV
  • Naive T cells
  • Recent thymic emigrants
  • TRECs

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Entrapment of recent thymic emigrants in lymphoid tissues from HIV-infected patients : Association with HIV cellular viral load. / Nokta, Mostafa A.; Li, Xiao Dong; Al-Harthi, Lena; Nichols, Joan; Pou, Anna; Asmuth, David; Landay, Alan; Pollard, Richard B.

In: AIDS, Vol. 16, No. 16, 08.11.2002, p. 2119-2127.

Research output: Contribution to journalArticle

Nokta, Mostafa A. ; Li, Xiao Dong ; Al-Harthi, Lena ; Nichols, Joan ; Pou, Anna ; Asmuth, David ; Landay, Alan ; Pollard, Richard B. / Entrapment of recent thymic emigrants in lymphoid tissues from HIV-infected patients : Association with HIV cellular viral load. In: AIDS. 2002 ; Vol. 16, No. 16. pp. 2119-2127.
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AU - Nokta, Mostafa A.

AU - Li, Xiao Dong

AU - Al-Harthi, Lena

AU - Nichols, Joan

AU - Pou, Anna

AU - Asmuth, David

AU - Landay, Alan

AU - Pollard, Richard B

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AB - Objective(s): Depletion of thymus derived naive T-cells is a feature of HIV infection. Here the impact of HIV infection on the compartmentalization of recent thymic emigrants of (RTE) and naive T-cells was examined. Methods: Peripheral blood mononuclear cells (PBMC) and lymphoid tissue (LT) from 43 HIV-infected patients and 12 controls were examined for RTE distribution by measuring coding joint T-cell receptor excisional circles (cjTREC) by PCR and naive and memory T-cell subsets and adhesion molecules (L-selection, LFA-1) by flow cytometry. Results: In HIV-infected patients, the RTE as quantified by cjTRECs in CD4 LT cells were significantly higher than in PBMC. Their values, however, were less than in control subjects, in both the LT and PBMC compartments. This was associated with an increase in L-selectin and LFA-1 expression on LT derived T cells. In PBMC, a significant positive relationship between TREC and naive CD4 cells and an inverse relationship between TREC and cellular viral load (CVL) was observed. Whereas in LT, there was a positive relationship between cjTREC and both naive CD4 cell percentage and CVL. Conclusions: Collectively, the data suggests that LT is a significant reservoir for RTE. The RTE appeared to be entrapped in LT from HIV-infected subjects. Such entrapment is probably a response to the high viral load in these tissues. These observations may partially explain the decline in RTE observed in the peripheral blood of HIV-infected patients, and the delay in recovery of naive cells in blood after initiation of HAART.

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