Entosis Is Induced by Glucose Starvation

Jens C. Hamann, Alexandra Surcel, Ruoyao Chen, Carolyn Teragawa, John Albeck, Douglas N. Robinson, Michael Overholtzer

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Entosis is a mechanism of cell death that involves neighbor cell ingestion. This process occurs in cancers and promotes a form of cell competition, where winner cells engulf and kill losers. Entosis is driven by a mechanical differential that allows softer cells to eliminate stiffer cells. While this process can be induced by matrix detachment, whether other stressors can activate entosis is unknown. Here, we find that entosis is induced in adherent cells by glucose withdrawal. Glucose withdrawal leads to a bimodal distribution of cells based on their deformability, where stiffer cells appear in a manner requiring the energy-sensing AMP-activated protein kinase (AMPK). We show that loser cells with high levels of AMPK activity are eliminated by winners through entosis, which supports winner cell proliferation under nutrient-deprived conditions. Our findings demonstrate that entosis serves as a cellular response to metabolic stress that enables nutrient recovery through neighbor cell ingestion.

Original languageEnglish (US)
Pages (from-to)201-210
Number of pages10
JournalCell Reports
Volume20
Issue number1
DOIs
StatePublished - Jul 5 2017

Fingerprint

Entosis
AMP-Activated Protein Kinases
Starvation
Nutrients
Glucose
Cell proliferation
Cell death
Formability
Recovery
Eating
Food
Physiological Stress

Keywords

  • AMPK
  • cannibalism
  • cell death
  • cell-in-cell
  • entosis
  • glucose starvation
  • myosin
  • tension

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hamann, J. C., Surcel, A., Chen, R., Teragawa, C., Albeck, J., Robinson, D. N., & Overholtzer, M. (2017). Entosis Is Induced by Glucose Starvation. Cell Reports, 20(1), 201-210. https://doi.org/10.1016/j.celrep.2017.06.037

Entosis Is Induced by Glucose Starvation. / Hamann, Jens C.; Surcel, Alexandra; Chen, Ruoyao; Teragawa, Carolyn; Albeck, John; Robinson, Douglas N.; Overholtzer, Michael.

In: Cell Reports, Vol. 20, No. 1, 05.07.2017, p. 201-210.

Research output: Contribution to journalArticle

Hamann, JC, Surcel, A, Chen, R, Teragawa, C, Albeck, J, Robinson, DN & Overholtzer, M 2017, 'Entosis Is Induced by Glucose Starvation', Cell Reports, vol. 20, no. 1, pp. 201-210. https://doi.org/10.1016/j.celrep.2017.06.037
Hamann JC, Surcel A, Chen R, Teragawa C, Albeck J, Robinson DN et al. Entosis Is Induced by Glucose Starvation. Cell Reports. 2017 Jul 5;20(1):201-210. https://doi.org/10.1016/j.celrep.2017.06.037
Hamann, Jens C. ; Surcel, Alexandra ; Chen, Ruoyao ; Teragawa, Carolyn ; Albeck, John ; Robinson, Douglas N. ; Overholtzer, Michael. / Entosis Is Induced by Glucose Starvation. In: Cell Reports. 2017 ; Vol. 20, No. 1. pp. 201-210.
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