Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells

Anandi Rajan, B. David Persson, Lars Frängsmyr, Annelie Olofsson, Linda Sandblad, Jyrki Heino, Yoshikazu Takada, A. Paul Mould, Lynn M. Schnapp, Jason Gall, Niklas Arnberg

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The enteric species F human adenovirus types 40 and 41 (HAdV-40 and -41) are the third most common cause of infantile gastroenteritis in the world. Knowledge about HAdV-40 and -41 cellular infection is assumed to be fundamentally different from that of other HAdVs since HAdV-40 and -41 penton bases lack the αV-integrin-interacting RGD motif. This motif is used by other HAdVs mainly for internalization and endosomal escape. We hypothesised that the penton bases of HAdV-40 and -41 interact with integrins independently of the RGD motif. HAdV-41 transduction of a library of rodent cells expressing specific human integrin subunits pointed to the use of laminin-binding α2-, α3- and α6-containing integrins as well as other integrins as candidate co-receptors. Specific laminins prevented internalisation and infection, and recombinant, soluble HAdV-41 penton base proteins prevented infection of human intestinal HT-29 cells. Surface plasmon resonance analysis demonstrated that HAdV-40 and -41 penton base proteins bind to α6-containing integrins with an affinity similar to that of previously characterised penton base:integrin interactions. With these results, we propose that laminin-binding integrins are co-receptors for HAdV-40 and -41.

Original languageEnglish (US)
Article number10019
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Human Adenoviruses
Laminin
Coinfection
Integrins
Infection
HT29 Cells
Surface Plasmon Resonance
Gastroenteritis
Libraries
Rodentia
Proteins

ASJC Scopus subject areas

  • General

Cite this

Rajan, A., Persson, B. D., Frängsmyr, L., Olofsson, A., Sandblad, L., Heino, J., ... Arnberg, N. (2018). Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells. Scientific Reports, 8(1), [10019]. https://doi.org/10.1038/s41598-018-28255-7

Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells. / Rajan, Anandi; Persson, B. David; Frängsmyr, Lars; Olofsson, Annelie; Sandblad, Linda; Heino, Jyrki; Takada, Yoshikazu; Mould, A. Paul; Schnapp, Lynn M.; Gall, Jason; Arnberg, Niklas.

In: Scientific Reports, Vol. 8, No. 1, 10019, 01.12.2018.

Research output: Contribution to journalArticle

Rajan, A, Persson, BD, Frängsmyr, L, Olofsson, A, Sandblad, L, Heino, J, Takada, Y, Mould, AP, Schnapp, LM, Gall, J & Arnberg, N 2018, 'Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells', Scientific Reports, vol. 8, no. 1, 10019. https://doi.org/10.1038/s41598-018-28255-7
Rajan, Anandi ; Persson, B. David ; Frängsmyr, Lars ; Olofsson, Annelie ; Sandblad, Linda ; Heino, Jyrki ; Takada, Yoshikazu ; Mould, A. Paul ; Schnapp, Lynn M. ; Gall, Jason ; Arnberg, Niklas. / Enteric Species F Human Adenoviruses use Laminin-Binding Integrins as Co-Receptors for Infection of Ht-29 Cells. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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